AUA Med 1 -- Biochemistry—Biotechniques—Questions With Complete Solutions

what is the purpose of Polymerase chain reaction (PCR)? correct answer: diagnostic tool to amplify a desired DNA fragment. What do you need for PCR? correct answer: Excess PRIMERS, Excess dNTPs, DNA polymerase (taq) What is the purpose of DNA polymerase ? correct answer: makes polymers of any molecules what is a chain reaction ? correct answer: a reaction that progress in an exponential way 2--4--6--8--10 why making copied of DNA ? correct answer: diagnosing infections, crime investigations, molecular biology, genetic research what is the purpose of primers? correct answer: needed to start the reaction , help identifying the portion of amplification What is the Process of PCR ? correct answer: denaturing , annealing , elongation What is denaturing ? correct answer: Heating the DNA to 95 to separate the strands What is annealing ? correct answer: Cooling the sample to 55 so that the primers bind to their target sequences what is elongation? correct answer: raising the temp to 72 so that the taq polymerase attach the dNTP's to replicate the sequence after each primer What is CRISPR-Cas9? correct answer: (cut and paste to edit DNA) Palindromic, cas9 cuts (cleaves) the DNA , Guide RNA (gRNA) recognize the sequence that need to be edited what is the meaning of blotting ? correct answer: detecting What are the 3 blotting procedures? correct answer: Southern, Northern , Western