BIO 471 Final (CORRECTLY ANSWERED)

A polylinker is a: correct answers synthetic DNA fragment that contains multiple restriction enzyme sites Which of the following statements about probes used in hybridization methods is TRUE? correct answers All of these statements about hybridization probes are true. A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of a pBR322 vector. The vector also contains a TetR gene that is not interrupted. After introducing the recombinant DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected? correct answers growth on tetracycline but not ampicillin containing media What did the X-ray diffraction patterns initially reveal about the DNA molecule? correct answers It is of uniform diameter and has a helical structure. Which of the following statements is true? correct answers The hydrogen bonding of cytosine to guanine is an example of complementary base pairing immunofluorescence methods can be carried out with antibodies that directly recognize the protein of interest. The indirect method is different because it requires that the antibody to the protein of interest be: correct answers detected by a second antibody that is attached to a fluorochrome. n the early 1900s, determining the chemical basis of heredity was difficult because chromosomes are composed of: correct answers DNA and protein. Although RNA is typically single-stranded, some RNA molecules, such as tRNA, have regions of base pairing. Which of the following pairings is possible correct answers . U-A Polar covalent bonds: correct answers All of these choices are correct. Which of the following is a difference between weak chemical bonds and covalent or ionic bonds? correct answers Weak chemical bonds are transient compared to covalent and ionic bonds. A deoxyribonucleotide is composed of a correct answers nitrogenous base, a deoxyribose, and a phosphate group The difference between a nucleotide and a nucleoside is the presence of which of the following correct answers a phosphate group Which of the following is an enzyme used in cloning that breaks covalent bonds? correct answers restriction endonuclease