BACE Review (Technical Skills & Applications) Question and Answer

Which of the following should not be used to disinfect an area that is potentially contaminated with DNA? - Alcohol Why are enzyme solutions always prepared using a buffered solvent at a specific pH? - A buffered solvent is resistant to fluctuations in pH because it is made with a combination of a weak acid and its conjugate base. Enzymes perform best in solutions that vary in pH. Which of the following statements best illustrates the reason for separation of DNA during gel electrophoresis? - DNA fragments are separated in the gel matrix based on their size. Which of the following should be included in a gel electrophoresis to determine the size of the DNA fragment on the gel? - Molecular Weight Markers Gels are used to separate pieces of RNA, DNA, and protein. What type of gel is typically used for protein electrophoresis? - Polyacrylamide A plasmid has three restriction sites for EcoRI. If the plasmid is treated EcoR1, how many fragments will be obtained? - 3 Which of the following can be used as a vehicle for delivering new DNA into a cell? - Plasmid, Lipid and Virus When developing a new PCR procedure, every variable needs to be tested to determine the optimum conditions for that specific reaction. Which of the statements best describes the best experimental design for optimizing the primer-annealing temperature? - Multiple reactions run with Primer, DNA, dNPT's, and a buffer concentration kept constant, only changing the annealing temperature in a range of 50 to 70 degrees celsius. Which of the following enzymes are used to amplify DNA in a Polymerase Chain Reaction (PCR)? - DNA Polymerase Heat-Stable DNA Polymerase - Polymerase Chain Reaction (PCR) Restriction Enzymes - Digesting DNA