WGU C785 Biochemistry Final Exam

WGU 785 Final Exam Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - CORRECT ANSWER His daughters would be carriers. This is x-link recessive. Autosomal: Dominant: - CORRECT ANSWER Autosomal: males and females equally affected. Dominant: non-carrier parents polymerase chain reaction (PCR) - CORRECT ANSWER The process of copying DNA in the lab. Uses Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 3 Steps of PCR - CORRECT ANSWER 1. Denaturation: DNA is heated to 95C to separate it. 2. Annealing: reaction is cooled to 50C; primers stick to the DNA you want to copy and add DNA polymerase. 3. Elongation: reaction heated to 70C and DNA polymerase, adding nucleotides building a new DNA strand.