DOPS
SKIN SPECIMEN TAKING
Leprosy BTA examination
A. Introduction
Ziehl-Neelsen (ZN) staining ( staining ) , also called recognized as dye bacteria
stand sour or often called BTA painting . In this paint contain substance color carbol -
fuchsin which is sour . Painting This First very initiated by two doctors Germany , Franz
Ziehl (1859-1926), a expert bacteria and Friedrich Neelsen (1854-1894), expert
pathology . ZN painting is dye bacteria typically used _ For organisms / bacteria stand
acids , especially Mycobacteria.
Bacteria This cause disease called skin _ as Hansen's leprosy or morbus. Slit skin
smear or skin smear is tissue inspection _ skin with method incisions and skin scrapings .
Result of smear skin (slit-skin smear) is used for the diagnosis and prognosis of leprosy.
The diagnosis of leprosy meets at least 1 of 3 signs cardinal namely (WHO, 2018 ):
Lost sensation in the affected lesion hypopigmentation
Thickening nerve peripheral accompanied with lost sensation and weakness
muscles on nerves related
Got bacteria stand acid on skin-slit smear examination . smear skin This is
procedure invasive , so needed aseptic action . _ specimen taken from the second
lobule ear , one lesion hypopigmentation .
B. Principle ZN staining
Mycobacterium sp own wall thick cells _ contains waxes of lipids and acids
mycolate that causes bacteria This difficult penetrated by paint normal .
ZN paint composition
ZN A: primer paint, contains Carbol fuchsin 1%, red paint dark in 5% soluble
phenol in lipid material like that of the walls Mycobacterium sp cells . Penetration
of this paint will made easy with exists helpful warm - up carbol fuchsin
penetrates lipid wall towards cytoplasm .
SKIN SPECIMEN TAKING
Leprosy BTA examination
A. Introduction
Ziehl-Neelsen (ZN) staining ( staining ) , also called recognized as dye bacteria
stand sour or often called BTA painting . In this paint contain substance color carbol -
fuchsin which is sour . Painting This First very initiated by two doctors Germany , Franz
Ziehl (1859-1926), a expert bacteria and Friedrich Neelsen (1854-1894), expert
pathology . ZN painting is dye bacteria typically used _ For organisms / bacteria stand
acids , especially Mycobacteria.
Bacteria This cause disease called skin _ as Hansen's leprosy or morbus. Slit skin
smear or skin smear is tissue inspection _ skin with method incisions and skin scrapings .
Result of smear skin (slit-skin smear) is used for the diagnosis and prognosis of leprosy.
The diagnosis of leprosy meets at least 1 of 3 signs cardinal namely (WHO, 2018 ):
Lost sensation in the affected lesion hypopigmentation
Thickening nerve peripheral accompanied with lost sensation and weakness
muscles on nerves related
Got bacteria stand acid on skin-slit smear examination . smear skin This is
procedure invasive , so needed aseptic action . _ specimen taken from the second
lobule ear , one lesion hypopigmentation .
B. Principle ZN staining
Mycobacterium sp own wall thick cells _ contains waxes of lipids and acids
mycolate that causes bacteria This difficult penetrated by paint normal .
ZN paint composition
ZN A: primer paint, contains Carbol fuchsin 1%, red paint dark in 5% soluble
phenol in lipid material like that of the walls Mycobacterium sp cells . Penetration
of this paint will made easy with exists helpful warm - up carbol fuchsin
penetrates lipid wall towards cytoplasm .
