Untitled-16 1 12/19/17 9:25
, Brief Contents
PA RT ON E PART FOUR
G EN E S , C H R O MOS OME S , AND GENETIC TECHNOLOGY AND
H ERE D IT Y GENOM ICS
1 Introduction to Genetics 1 20 Recombinant DNA Technology 454
2 Mitosis and Meiosis 14 21 Genomic Analysis 485
3 Mendelian Genetics 36 22 Applications of Genetic Engineering and
4 Extensions of Mendelian Genetics 62 Biotechnology 521
5 Chromosome Mapping in Eukaryotes 94
6 Genetic Analysis and Mapping in Bacteria and PART FIVE
Bacteriophages 123
GENETIC ANALYSIS OF ORGA NISM S
7 Sex Determination and Sex Chromosomes 151 AND POPULATIONS
8 Chromosomal Mutations: Variation in Number
and Arrangement 171 23 Developmental Genetics 555
9 Extranuclear Inheritance 196 24 Cancer Genetics 579
25 Quantitative Genetics and Multifactorial
Traits 599
PA RT TWO 26 Population and Evolutionary Genetics 621
D N A : ST R U C T UR E , R E P LIC ATION,
A N D O R G A N IZ AT ION
SPECIAL TOPICS IN M OD E RN
10 DNA Structure and Analysis 213
GENETICS
11 DNA Replication and Recombination 238
1 CRISPR-Cas and Genome Editing 649
12 DNA Organization in Chromosomes 263
2 DNA Forensics 661
3 Genomics and Precision Medicine 672
PA RT T HR E E
4 Genetically Modified Foods 683
G EN E E X P R E S S IO N A ND IT S 5 Gene Therapy 695
R EG U L AT I O N
6 Advances in Neurogenetics: The Study of
13 The Genetic Code and Transcription 283 Huntington Disease 710
14 Translation and Proteins 312
15 Gene Mutation, DNA Repair, and Appendix A Selected Readings A-1
Transposition 340
Appendix B Answers to Selected Problems B-1
16 Regulation of Gene Expression in Bacteria 373
Glossary G-1
17 Transcriptional Regulation in Eukaryotes 393
Credits C-1
18 Posttranscriptional Regulation in
Eukaryotes 413 Index I-1
19 Epigenetic Regulation of Gene Expression 433
CVR_KLUG4718_12_SE_BEP.indd 2-3
,Nobel Prizes Awarded for Research in Genetics or Genetics-Related Areas
Year Recipients Nobel Prize Discovery/Research Topic
2017 J. C. Hall Physiology or Identification of the genes and molecular mechanisms
M. Rosbash Medicine that regulate circadian rhythms
M. W. Young
2015 T. Lindahl Chemistry Mechanistic studies of DNA repair
P. Modrich
A. Sancar
2012 J. B. Gurdon Physiology or Differentiated cells can be reprogrammed to become
S. Yamanaka Medicine pluripotent
2009 V. Ramakrishnan Chemistry Structure and function of the ribosome
T. A. Steitz
A. E. Yonath
2009 E. H. Blackburn Physiology or The nature and replication of the DNA of telomeres,
C. W. Greider Medicine and the discovery of the telomere-replenishing ribonu-
J. W. Szostak cleoprotein enzyme telomerase
2008 O. Shimomura Chemistry Discovery and development of a genetically encoded
M. Chalfie fluorescent protein as an in vivo marker of gene
R. Y. Tsien expression
2007 M. R. Capecchi Physiology or Gene-targeting technology essential to the creation
M. J. Evans Medicine of knockout mice serving as animal models of human
O. Smithies disease
2006 R. D. Kornberg Chemistry Molecular basis of eukaryotic transcription
2006 A. Z. Fire Physiology or Gene silencing using RNA interference (RNAi)
C. C. Mello Medicine
2004 A. Ciechanover Chemistry Regulation of protein degradation by the proteasome
A. Hershko
I. Rose
2002 S. Brenner Physiology or Genetic regulation of organ development and pro-
H. R. Horvitz Medicine grammed cell death (apoptosis)
J. E. Sulston
2001 L. H. Hartwell Physiology or Genes and regulatory molecules controlling the cell
T. Hunt Medicine cycle
P. M. Nurse
1999 G. Blobel Physiology or Genetically encoded amino acid sequences in proteins
Medicine that guide their cellular transport
1997 S. B. Prusiner Physiology or Prions—a new biological principle of infection
Medicine
1995 E. B. Lewis Physiology or Genetic control of early development in Drosophila
C. NÜsslein-Volhard Medicine
E. Wieschaus
1993 R. J. Roberts Physiology or RNA processing of split genes
P. A. Sharp Medicine
K. B. Mullis Chemistry Development of polymerase chain reaction (PCR) and
M. Smith site-directed mutagenesis (SDM)
1989 J. M. Bishop Physiology or Role of retroviruses and oncogenes in cancer
H. E. Varmus Medicine
T. R. Cech Chemistry Ribozyme function during RNA splicing
S. Altman
1987 S. Tonegawa Physiology or Genetic basis of antibody diversity
Medicine
12/1/17 1:02 P
, Year Recipients Nobel Prize Discovery/Research Topic
1985 M. S. Brown Physiology or Genetic regulation of cholesterol metabolism
J. L. Goldstein Medicine
1983 B. McClintock Physiology or Mobile genetic elements in maize
Medicine
1982 A. Klug Chemistry Crystalline structure analysis of significant complexes,
including tRNA and nucleosomes
1980 P. Berg Chemistry Development of recombinant DNA and DNA sequenc-
W. Gilbert ing technology
F. Sanger
1978 W. Arber Physiology or Recombinant DNA technology using restriction endo-
D. Nathans Medicine nuclease technology
H. O. Smith
1976 B. S. Blumberg Physiology or Elucidation of the human prion-based diseases, kuru
D. C. Gajdusek Medicine and Creutzfeldt-Jakob disease
1975 D. Baltimore Physiology or Molecular genetics of tumor viruses
R. Dulbecco Medicine
H. M. Temin
1972 G. M. Edelman Physiology or Chemical structure of immunoglobulins
R. R. Porter Medicine
C. B. Anfinsen Chemistry Relationship between primary and tertiary structure
of proteins
1970 N. Borlaug Peace Prize Genetic improvement of Mexican wheat
1969 M. Delbrück Physiology or Replication mechanisms and genetic structure of
A. D. Hershey Medicine bacteriophages
S. E. Luria
1968 H. G. Khorana Physiology or For their interpretation of the genetic code and its
M. W. Nirenberg Medicine function during protein synthesis
R. W. Holley
1966 P. F. Rous Physiology or Viral induction of cancer in chickens
Medicine
1965 F. Jacob Physiology or Genetic regulation of enzyme synthesis in bacteria
A. M. Lwoff Medicine
J. L. Monod
1962 F. H. C. Crick Physiology or Double helical model of DNA
J. D. Watson Medicine
M. H. F. Wilkins
J. C. Kendrew Chemistry Three-dimensional structure of globular proteins
M. F. Perutz
1959 A. Kornberg Physiology or Biological synthesis of DNA and RNA
S. Ochoa Medicine
1958 G. W. Beadle Physiology or Genetic control of biochemical processes
E. L. Tatum Medicine
J. Lederberg Physiology or Genetic recombination in bacteria
Medicine
F. Sanger Chemistry Primary structure of proteins
1954 L. C. Pauling Chemistry Alpha helical structure of proteins
1946 H. J. MÜller Physiology or X-ray induction of mutations in Drosophila
Medicine
1933 T. H. Morgan Physiology or Chromosomal theory of inheritance
Medicine
1930 K. Landsteiner Physiology or Discovery of human blood groups
Medicine
CVR_KLUG4718_12_SE_BEP.indd 4-5
, Brief Contents
PA RT ON E PART FOUR
G EN E S , C H R O MOS OME S , AND GENETIC TECHNOLOGY AND
H ERE D IT Y GENOM ICS
1 Introduction to Genetics 1 20 Recombinant DNA Technology 454
2 Mitosis and Meiosis 14 21 Genomic Analysis 485
3 Mendelian Genetics 36 22 Applications of Genetic Engineering and
4 Extensions of Mendelian Genetics 62 Biotechnology 521
5 Chromosome Mapping in Eukaryotes 94
6 Genetic Analysis and Mapping in Bacteria and PART FIVE
Bacteriophages 123
GENETIC ANALYSIS OF ORGA NISM S
7 Sex Determination and Sex Chromosomes 151 AND POPULATIONS
8 Chromosomal Mutations: Variation in Number
and Arrangement 171 23 Developmental Genetics 555
9 Extranuclear Inheritance 196 24 Cancer Genetics 579
25 Quantitative Genetics and Multifactorial
Traits 599
PA RT TWO 26 Population and Evolutionary Genetics 621
D N A : ST R U C T UR E , R E P LIC ATION,
A N D O R G A N IZ AT ION
SPECIAL TOPICS IN M OD E RN
10 DNA Structure and Analysis 213
GENETICS
11 DNA Replication and Recombination 238
1 CRISPR-Cas and Genome Editing 649
12 DNA Organization in Chromosomes 263
2 DNA Forensics 661
3 Genomics and Precision Medicine 672
PA RT T HR E E
4 Genetically Modified Foods 683
G EN E E X P R E S S IO N A ND IT S 5 Gene Therapy 695
R EG U L AT I O N
6 Advances in Neurogenetics: The Study of
13 The Genetic Code and Transcription 283 Huntington Disease 710
14 Translation and Proteins 312
15 Gene Mutation, DNA Repair, and Appendix A Selected Readings A-1
Transposition 340
Appendix B Answers to Selected Problems B-1
16 Regulation of Gene Expression in Bacteria 373
Glossary G-1
17 Transcriptional Regulation in Eukaryotes 393
Credits C-1
18 Posttranscriptional Regulation in
Eukaryotes 413 Index I-1
19 Epigenetic Regulation of Gene Expression 433
CVR_KLUG4718_12_SE_BEP.indd 2-3
,Nobel Prizes Awarded for Research in Genetics or Genetics-Related Areas
Year Recipients Nobel Prize Discovery/Research Topic
2017 J. C. Hall Physiology or Identification of the genes and molecular mechanisms
M. Rosbash Medicine that regulate circadian rhythms
M. W. Young
2015 T. Lindahl Chemistry Mechanistic studies of DNA repair
P. Modrich
A. Sancar
2012 J. B. Gurdon Physiology or Differentiated cells can be reprogrammed to become
S. Yamanaka Medicine pluripotent
2009 V. Ramakrishnan Chemistry Structure and function of the ribosome
T. A. Steitz
A. E. Yonath
2009 E. H. Blackburn Physiology or The nature and replication of the DNA of telomeres,
C. W. Greider Medicine and the discovery of the telomere-replenishing ribonu-
J. W. Szostak cleoprotein enzyme telomerase
2008 O. Shimomura Chemistry Discovery and development of a genetically encoded
M. Chalfie fluorescent protein as an in vivo marker of gene
R. Y. Tsien expression
2007 M. R. Capecchi Physiology or Gene-targeting technology essential to the creation
M. J. Evans Medicine of knockout mice serving as animal models of human
O. Smithies disease
2006 R. D. Kornberg Chemistry Molecular basis of eukaryotic transcription
2006 A. Z. Fire Physiology or Gene silencing using RNA interference (RNAi)
C. C. Mello Medicine
2004 A. Ciechanover Chemistry Regulation of protein degradation by the proteasome
A. Hershko
I. Rose
2002 S. Brenner Physiology or Genetic regulation of organ development and pro-
H. R. Horvitz Medicine grammed cell death (apoptosis)
J. E. Sulston
2001 L. H. Hartwell Physiology or Genes and regulatory molecules controlling the cell
T. Hunt Medicine cycle
P. M. Nurse
1999 G. Blobel Physiology or Genetically encoded amino acid sequences in proteins
Medicine that guide their cellular transport
1997 S. B. Prusiner Physiology or Prions—a new biological principle of infection
Medicine
1995 E. B. Lewis Physiology or Genetic control of early development in Drosophila
C. NÜsslein-Volhard Medicine
E. Wieschaus
1993 R. J. Roberts Physiology or RNA processing of split genes
P. A. Sharp Medicine
K. B. Mullis Chemistry Development of polymerase chain reaction (PCR) and
M. Smith site-directed mutagenesis (SDM)
1989 J. M. Bishop Physiology or Role of retroviruses and oncogenes in cancer
H. E. Varmus Medicine
T. R. Cech Chemistry Ribozyme function during RNA splicing
S. Altman
1987 S. Tonegawa Physiology or Genetic basis of antibody diversity
Medicine
12/1/17 1:02 P
, Year Recipients Nobel Prize Discovery/Research Topic
1985 M. S. Brown Physiology or Genetic regulation of cholesterol metabolism
J. L. Goldstein Medicine
1983 B. McClintock Physiology or Mobile genetic elements in maize
Medicine
1982 A. Klug Chemistry Crystalline structure analysis of significant complexes,
including tRNA and nucleosomes
1980 P. Berg Chemistry Development of recombinant DNA and DNA sequenc-
W. Gilbert ing technology
F. Sanger
1978 W. Arber Physiology or Recombinant DNA technology using restriction endo-
D. Nathans Medicine nuclease technology
H. O. Smith
1976 B. S. Blumberg Physiology or Elucidation of the human prion-based diseases, kuru
D. C. Gajdusek Medicine and Creutzfeldt-Jakob disease
1975 D. Baltimore Physiology or Molecular genetics of tumor viruses
R. Dulbecco Medicine
H. M. Temin
1972 G. M. Edelman Physiology or Chemical structure of immunoglobulins
R. R. Porter Medicine
C. B. Anfinsen Chemistry Relationship between primary and tertiary structure
of proteins
1970 N. Borlaug Peace Prize Genetic improvement of Mexican wheat
1969 M. Delbrück Physiology or Replication mechanisms and genetic structure of
A. D. Hershey Medicine bacteriophages
S. E. Luria
1968 H. G. Khorana Physiology or For their interpretation of the genetic code and its
M. W. Nirenberg Medicine function during protein synthesis
R. W. Holley
1966 P. F. Rous Physiology or Viral induction of cancer in chickens
Medicine
1965 F. Jacob Physiology or Genetic regulation of enzyme synthesis in bacteria
A. M. Lwoff Medicine
J. L. Monod
1962 F. H. C. Crick Physiology or Double helical model of DNA
J. D. Watson Medicine
M. H. F. Wilkins
J. C. Kendrew Chemistry Three-dimensional structure of globular proteins
M. F. Perutz
1959 A. Kornberg Physiology or Biological synthesis of DNA and RNA
S. Ochoa Medicine
1958 G. W. Beadle Physiology or Genetic control of biochemical processes
E. L. Tatum Medicine
J. Lederberg Physiology or Genetic recombination in bacteria
Medicine
F. Sanger Chemistry Primary structure of proteins
1954 L. C. Pauling Chemistry Alpha helical structure of proteins
1946 H. J. MÜller Physiology or X-ray induction of mutations in Drosophila
Medicine
1933 T. H. Morgan Physiology or Chromosomal theory of inheritance
Medicine
1930 K. Landsteiner Physiology or Discovery of human blood groups
Medicine
CVR_KLUG4718_12_SE_BEP.indd 4-5
