Portage Learning BIO 171- Microbiology Lab Notebook

Title: KD01 Introduction to medical microbiology & lab notebook procedures Course Objectives: Cultivation ofsamples: growth conditions and equipment used Identification of samples: biochemical assays & tests available Evaluation ofsamples: microscopy- visualization and key characteristics ofspecimens Basic Equipment: Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take minutes to sterilize & hot dry air which will take hours; before opening make sure itis depressurized and will have very hot air. Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes - Shaker incubator 37°C shakes culture & rotates to aerateliquid nutrient broth Visualizing: microscopy Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term Lab Safety 1. Never eat or drink in the lab: contamination risks 2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue, green),thermal, or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills Portage Learning / BIOD 171 Microbiology Lab Notebook (2) (2) (1)-5 2021 3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria Outline of how to set up Notebook Objective: To establish an organized template for keeping experimentalrecords, procedures, and results. Procedure: 1. This is where each step of a given protocol is recorded 2. Be sure to clearly indicate any deviations that may occur during the experiment- put them in red Notes: Additional (helpful) information placed here Results: A summary of the final outcome of the experiment should be described here