BIOS 242 Week 6 Lab 2; Bacteria of Respiratory System

Lab12:BacteriaoftheRespiratorySystem Introduction: Therespiratorysystemisdividedintotwoparts:theupperandlowerrespiratorysystems.Thelowerrespiratorysystemisanaxenicenvironmentbecauseof the cilia that lines the respiratory tract, alveolar macrophages and IgA antibodies found in the mucus. The upper part harbors a variety of microbiotasuch as Gram-negative bacteria Hemophilus, Gram-positive bacteria such as Staphylococcus aureus and diphtheroids that can cause opportunisticinfectionsleading toinfectionlike pneumonia anddiphtheria……. Continue 1. Alphahemolysis: 2. Betahemolysis: 3. Gammahemolysis: Materials: LiquidculturesofB.subtilis,E.coli,S.epidermidisandS.marcescens,BAPplates,Incinerators,Metalloops Method: 1. ObtainliquidculturesofS. epidermidis,E.coli,B.subtilis andS.marcescensaswellastwoBAPplatespergroupoffourstudents. 2. TakeoneBAPplateanddivideitinthreeparts. 3. Labeleachpartcorrectlywiththenameofthebacteria.Leavethethirdareaempty. 4. TakesecondBAPplateanddivideitinthreeparts. 5. Labeleachpartcorrectlywiththenameofthebacteria.Leavethethirdareaempty. 6. Usingasepticculturetransfertechniques,streaktheculturesonBAPplates. 7. Let the culturesgrow overnight at 370C. 8. ObservethegrowthandcompleteLabReport. Purpose:Describethepurposeofthislab. 1. Isbloodagarselectiveordifferential?Brieflyexplain. 2. IstheGramstainofsignificantimportanceinidentifyingtheorganismsstudiedinthisexercise?Explain. 3. ListsomediseasesofupperrespiratorysystemthatyoulearnedinlecturethatcanhelpyouidentifythecausativemicroorganismusingBAPplates. Observation:Completethefollowingtable.ObtaintheresultsofGramreaction,morphologyandarrangementfromlabs4and5.Reviewliteraturetofindreaction ofS. aureus.