Unit 6 learning aim B
Produce a plan for an investigative project based on the proposal
Project timeline
Aim: assess the effects of caffeine on the heart rate of daphnia
Hypothesis: The concentration of caffeine will affect the heart rate of daphnia
accordingly which means if the caffeine concentration increases the heart rate
of daphnia will increase same goes for the opposite. This is because daphnia is
a stimulant and it’s known to raise the heart rate of some species.
Method
Remove 1 Daphnia with a pipette and place it in a cavity slide under a
microscope. Dab around the Daphnia with a tissue to remove the pond
water and replace it with drops of caffeine solution (e.g., 0.1M).
Leave the Daphnia for 5 minutes to acclimatise and then observe & count
(using a counter) the heart rate under the microscope for 30 seconds
(multiply the number by 2 to calculate beats per minute).
Repeat this for measurements across 5 different caffeine concentrations
(e.g., 0.2M, 0.3M, 0.4M and 0.5M). Repeats can then be carried out with
two other Daphnia.
Week Monday lesson Tuesday lesson
Thursday Milestones
10:35-12:35 13:25-14:25 lesson and
13:25-14:55 achievements
07/03/2022 In this lesson, the In this lesson, In this lesson, Tracking the
trial the calculations the outcomes data and
experiment/practi and results were were outcomes and
cal was done. This recorded along checked, and any new
helped me with the any errors or findings.
understand the method mistakes Presenting
possible errors development were fixed. In and analysing
that could occur and any other addition, any data
when doing the graphs and plotting found.
real one and tables that graphs were
prepare a plan to needed to be made.
fix or avoid them. constructed.
, 14/03/2022 The planned Some research The recorded Doing the
experiment was was done to find results were experiment
done in this out how to checked and and recording
lesson. Every plan check the calculated to results and
and preparation reliability of an make sure making sure
were applied experiment. that the it's as
accordingly in this results are accurate and
given time. reliable. precise as
possible.
21/03/2022 In this lesson, I In this lesson, I I finished the Making sure
compared my compared my conclusion that the
results with any results with my and prepared results
found published classmates’ to do the recorded are
data and kept work to have a report and reliable and
working on the better assignment precise. Start
conclusion conclusion on work. doing the
the experiment. report based
on the
conclusion
and other
aspects of the
experiment.
Possible problems and contingencies
One of the problems that could occur are:
Calculating enough time necessary to do the experiment- could be a problem
as we didn’t have a way to calculate how long will the experiment take and if
problems occur how long could it take to solve them. the experiment time was
set based on an estimate.
The wrong approach could be taken to carry out the experiment- this could
lead to imprecise or inaccurate results, as by mistake the wrong equipment or
wrong amount of concentration could be used and could lead to wrong
readings.
Lab access: as the lab is used by the college someone else might be using it at
the time that we want to use it. To prevent this the lab could be booked for
our use or another lab could be used to carry out the experiment.
Produce a plan for an investigative project based on the proposal
Project timeline
Aim: assess the effects of caffeine on the heart rate of daphnia
Hypothesis: The concentration of caffeine will affect the heart rate of daphnia
accordingly which means if the caffeine concentration increases the heart rate
of daphnia will increase same goes for the opposite. This is because daphnia is
a stimulant and it’s known to raise the heart rate of some species.
Method
Remove 1 Daphnia with a pipette and place it in a cavity slide under a
microscope. Dab around the Daphnia with a tissue to remove the pond
water and replace it with drops of caffeine solution (e.g., 0.1M).
Leave the Daphnia for 5 minutes to acclimatise and then observe & count
(using a counter) the heart rate under the microscope for 30 seconds
(multiply the number by 2 to calculate beats per minute).
Repeat this for measurements across 5 different caffeine concentrations
(e.g., 0.2M, 0.3M, 0.4M and 0.5M). Repeats can then be carried out with
two other Daphnia.
Week Monday lesson Tuesday lesson
Thursday Milestones
10:35-12:35 13:25-14:25 lesson and
13:25-14:55 achievements
07/03/2022 In this lesson, the In this lesson, In this lesson, Tracking the
trial the calculations the outcomes data and
experiment/practi and results were were outcomes and
cal was done. This recorded along checked, and any new
helped me with the any errors or findings.
understand the method mistakes Presenting
possible errors development were fixed. In and analysing
that could occur and any other addition, any data
when doing the graphs and plotting found.
real one and tables that graphs were
prepare a plan to needed to be made.
fix or avoid them. constructed.
, 14/03/2022 The planned Some research The recorded Doing the
experiment was was done to find results were experiment
done in this out how to checked and and recording
lesson. Every plan check the calculated to results and
and preparation reliability of an make sure making sure
were applied experiment. that the it's as
accordingly in this results are accurate and
given time. reliable. precise as
possible.
21/03/2022 In this lesson, I In this lesson, I I finished the Making sure
compared my compared my conclusion that the
results with any results with my and prepared results
found published classmates’ to do the recorded are
data and kept work to have a report and reliable and
working on the better assignment precise. Start
conclusion conclusion on work. doing the
the experiment. report based
on the
conclusion
and other
aspects of the
experiment.
Possible problems and contingencies
One of the problems that could occur are:
Calculating enough time necessary to do the experiment- could be a problem
as we didn’t have a way to calculate how long will the experiment take and if
problems occur how long could it take to solve them. the experiment time was
set based on an estimate.
The wrong approach could be taken to carry out the experiment- this could
lead to imprecise or inaccurate results, as by mistake the wrong equipment or
wrong amount of concentration could be used and could lead to wrong
readings.
Lab access: as the lab is used by the college someone else might be using it at
the time that we want to use it. To prevent this the lab could be booked for
our use or another lab could be used to carry out the experiment.
