Current Research Topics in Applied Microbiology and Microbial Biotechnology.

Current Research Topics in Applied Microbiology and Microbial Biotechnology. Contents Introduction ......................................................................................................................v Agriculture, Soil, Forest Microbiology.........................................................................1 Anti-oxidative stress enzymes in Pleurotus ostreatus.............................................................3 Beauveria bassiana mutants display a different protein profile in comparison with parental strain ..........................................................................................................................8 Culturable microbial populations in a vineyard soil under different management regimes: Influence on spontaneous must fermentation .........................................................12 Diagnosis and association of Olpidium bornovanus and MNSV with vine decline of melon in Honduras ................................................................................................................16 Effect of nitroaromatic compounds on the growth of potted plants ......................................24 Effects of cattle grazing, trampling and excrement deposition on microbial nitrogen transformations in upland soil ...............................................................................................29 Evaluation of Crude Glycerol from Biodiesel Production as a plant pathogen control agent ......................................................................................................................................33 Extended nutrient limitation influences Ralstonia solanacearum survival in natural water microcosms..................................................................................................................38 Genetic characterization of Plutella xylostella with resistance to formulates of Bacillus thuringiensis at laboratory and field level ...............................................................42 Hemicelluloses: from wood to the fermenter ........................................................................46 Identification and characterization of a Cry7-like protein of Bacillus thuringiensis GM-33 strain holotype for subsp. monterrey ........................................................................51 Identification of actinomycetes with antifungal activity isolated from soil amended with composts........................................................................................................................55 Identification of Mycobacterium sp. as Alfalfa endophytes using 16s rRNA gene sequence analysis...................................................................................................................59 Identification of the respiratory chain of Armillaria mellea (A.m.) in mushroom state and cultured in vitro...............................................................................................................64 Isolation and identification of sulphur-oxidizing bacteria from composted two-phase olive mill waste amended with elemental sulphur.................................................................69 Manipulating the growth of bold and small grains in the ear of Triticum aestivum by salicylhydroxamic acid..........................................................................................................74 New hosts for the enterobacterial phytopathogen Erwinia persicina....................................78 viii Production and Evaluation of Polyclonal Antisera for Detection of Ralstonia solanacearum ........................................................................................................................83 Survival of Erwinia amylovora in rain water at low temperatures........................................88 The addition of copper sulphate to a non-selective medium improves the recovery of plant associated bacteria: Erwinia amylovora as a model .....................................................92 The effects of various organic wastes applied into eroded soil on dehydrogenase enzyme activity......................................................................................................................97 Use of vinasses in the control of fungi phytopathogens ......................................................102 Utilization of organic and mineral amendments to control potato bacterial wilt disease....109 Viability of culturable soil microorganisms during freeze storage......................................114 Analytical and Imaging Techniques. Microscopy ...................................................119 Characterization of Cell Walls from Mucoralean Fungi by Biochemical Composition, Transmission Electron Microscopy and X-Ray Microanalysis ...........................................121 Comparative analysis of different microbial techniques of quantification applied to anaerobic digestion..............................................................................................................126 Comparison of different analytical processes for patulin determination in apple juice ......131 Electroimmunoassay for detection of bacterial cells...........................................................136 Fine structure of wild type and mit- mutants of the yeast Saccharomyces cerevisiae.........140 Study of necessary pre-treatment for application of microbial techniques of quantification to high content in solids samples..................................................................145 Environmental, Marine, Aquatic Microbiology. Geomicrobiology.......................151 A preliminar survey on the fungi of Doña Trinidad Cave, Ardales, Malaga, Spain ...........153 A survey of microorganisms related to the biodeterioration of prehistoric paintings in natural shelters from Aragon (Spain) ..................................................................................157 An Investigation on Heterotrophic and Pathogenic Gastrointestinal Bacteria in Otamiri River, Nigeria.........................................................................................................162 Assessment of the performance of porphyrin derivatives as photosensitizers for the inactivation of bacterial endospores ....................................................................................166 Biodegradation of aromatic contaminants present in industrial production waste waters by Trichosporon cutaneum ......................................................................................170 Biodegradation of exogenous DNA by bio-products used in domestic sewage treatment..............................................................................................................................176 Biotechnological Potential of Phanerochaete chrysosporium UCP 963 and Cunninghamella elegans UCP 596 in the Copper and Zinc Removal ................................180 ix Cadmium toxicity on Cunninghamella elegans: Ultrastructural damage and actin cytoskeleton alterations .......................................................................................................184 Comparative analyses of microorganisms from different high-temperature volcanic environments .......................................................................................................................188 Composition of halophilic bacteria survived in bioaerosol .................................................193 Control of Legionella pneumophila by disinfectants. Effects of sodium hypochlorite against persistent strains ......................................................................................................198 Degradation of phenols by Fusarium moniliforme..............................................................203 Degradative potential of marine bacterial isolates from the aquatic plant Posidonia oceanica...............................................................................................................................208 Development of new primer systems for the detection of the polyphosphate kinase gene in activated sludge.......................................................................................................213 Effect of Phenanthrene on the Germination, Radial Growth and Chitin and Chitosan production by Cunninghamella elegans Lendner................................................................219 Effect of Pyrene on the growth of Rhodotorula sp..............................................................223 Effect of the introduction of an anaerobic phase on the protozoa community of an SBR used for biodecolorization of an azo dye ....................................................................228 Effect of water dilution and nutrient supplements (wood ash, urea and poultry droppings) on biogas production from brewers spent grain ................................................232 Evaluation of the impact of two aquaculture systems on bacterial communities of the estuarine system Ria de Aveiro ...........................................................................................236 Evaluation of the microbial diversity in anaerobic reactors fed with sucrose applied to hydrogen production............................................................................................................240 Evidence of a bimodal effect on Saccharomyces cerevisiae UE-ME3 by vanadium (V) stress – a dual response to different V2O5 medium concentration detected in the rate growth, GSH/GSSG, G6PD, CAT T and GR enzymatic activities .....................................245 Factorial design applied to biosurfactant production by Chromobacterium violaceum......249 Formation of Biofilms and Production of Enzymes by Bacillus subtilis on Surfaces of Polyethylene Terephtalate Simulating Degradation ............................................................254 Glutamylcysteine ligase gene of the ciliated protozoan Tetrahymena thermophila: A potential tool for pollution monitoring ................................................................................259 Identification of culturable psychrophilic yeasts isolated from sediments and melt waters of the Calderone Glacier (Italy) ...............................................................................264 In situ assessment of drinking water biostability using nascent reference biofilm ATRFTIR fingerprint ..................................................................................................................268 Influence of light: dark cycle in the cellular composition of Nannochloropsis gaditana ...273 x Isolation and Identification of Bacteria in the Anammox Activated Sludge.......................278 Keratinolytic activity of Streptomyces sp isolated of poultry processing plant...................282 Microbial characterization of Linear Alkylbenzene Sulfonate degradation in fixed bed anaerobic reactor .................................................................................................................286 Microbial communities from caves with paleolithic paintings............................................291 Microbial communities in different volcanic environments from Canary Islands (Spain) .................................................................................................................................295 Microbial diversity in Chromobacterium violaceum determined by 16S rRNA gene analysis ................................................................................................................................300 Microbial Pandora’s box : Interactions of free living protozoa with human pathogenic bacteria ................................................................................................................................304 Microbiological resistance of optical sights for civilian and military use...........................308 Molecular and phylogenetic analysis on bacterial strains isolated from a PAHs wastewater treatment plant ..................................................................................................313 Monitoring of Bacterial Diversity in Relation to PHA Storage Capacity in an Anaerobic/Aerobic Activated Sludge SBR System.............................................................317 Potential stage in wastewater treatment for generation of bioelectricity using MFC..........322 Production of Bioelectricity from Wastewater using stacked Microbial Fuel Cells ...........327 Seasonal dynamics of bacterial population degrading dimethylarsenic acid in Lake Kahokugata..........................................................................................................................333 Sewage bacteriophage photoinactivation by porphyrins immobilized in solid matrixes ....338 The DGGE technique and 16S rDNA clone libraries analysis as a microbiological indicator of soil degradation................................................................................................342 The Testate lobose amoebae in the wastewater treatment...................................................347 Treatment of linear alkylbenzene sulfonate in mesophilic anaerobic sequencing batch reactor..................................................................................................................................352 Food Microbiology......................................................................................................357 A simple method for simultaneously isolating mitDNA and virus dsRNA from wine yeasts ...................................................................................................................................359 Antioxidant substance production by the transformation of sweet potato using Aspergillus niger .................................................................................................................363 Biocontrol of Aspergillus ochraceus by yeasts ...................................................................368 Characterization of killer wine yeasts from spontaneous must fermentation in six wine producing zones of southwestern Spain ..............................................................................373 xi Conjugated linoleic acid: a multifunctional nutraceutical from the rumen .........................378 Effect of the baking process on the reduction of ochratoxin A in wheat flour....................382 Entrapment by Ca-Alginate Immobilized Yeast Cells for Dried Longan Wine Production............................................................................................................................386 Fungal spoilage in corn .......................................................................................................392 Isolation, phenotypic and genotypic characterization of quinolone-resistant Salmonella enterica strains isolated from foods and water .................................................397 Microbiology stability of wine from Castilla la Mancha.....................................................401 Occurrence of mycobiota in swine feed ..............................................................................407 Thermal inactivation of Escherichia coli and coliform in Oaxaca cheese curd during a simulated kneading process.................................................................................................412 Using food industry wastes for producing valuable materials: Skin of eggplant for wool dyeing .........................................................................................................................416 Industrial Microbiology. Future Bioindustries........................................................423 Application of Biotechnology in Petroleum Industry - Microbial Enhanced Oil Recovery..............................................................................................................................425 Development of biotechnological processes using glycerol from biodiesel production .....429 Effect of different physiological stress on flocculation and fermentative capacity of Saccharomyces cerevisiae in lager beer ..............................................................................434 Evaluation of the β-glucanolytic enzyme complex of Trichoderma harzianum Rifai for the production of gluco-oligosaccharide fragments by enzymatic hydrolysis of 1,3;1,6-β-D-glucans.............................................................................................................438 Growth of Kluyveromyces marxianus yeasts strains in deproteined whey obtained from dairy industry ..............................................................................................................442 Isolation and characterization of α-glucosidase — a thermostable intracellular enzyme from Aspergillus carbonarius var (bainer) Thom IMI .........................................445 Purification and biochemical characterization of an extracellular β-glucosidase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb. :Fr.) Rehm.............................448 Relationships between light-treated cultures and lactose content in yogurt........................456 The use of ScCO2 for the extraction of LPS from S. enterica subsp. PCM 2266................460 Use The Solid Fermentation as a New and Alternative Way for Xylitol Bioproduction ....465 Xylanase and cellulase free xylanase preparations from microscopic fungi isolated in the South Caucasus..........................................................................................................470 Xylose from Eucalyptus globulus wood as a raw material for bioethanol production........475 xii Medical Microbiology. Pharmaceutical Microbiology............................................481 β-lactam Resistance in Escherichia coli Isolates from Raptors in Spain ............................483 Characterization and molecular epidemiology of Enterobacter cloacae clinical isolates producing extended-spectrum β-lactamases...........................................................487 Detection of Porphyromonas gingivalis, Tanaerella forsythensis and Streptococcus intermedius in dental plaque and saliva in young Children by Multiplex PCR ..................493 Herbal Antibacterial Liquid Soap Development against Bacterial Skin Diseases ..............497 Importance of High Levels Detention by Enterococcus Resistance (HLR) to Aminoglycosides in the Treatment of Serious Infections from Hospital ............................501 In vitro mechanism of xylitol action against Staphylococcus aureus ATCC 25923 ...........505 Incidence and resistance profile of Cedecea sp. isolated from a hospital ...........................510 Inhibition by doxorubicin of anti-ROS enzymes superoxide dismutase and catalase in Salmonella typhimurium......................................................................................................515 Secondary metabolites produced by endophytic fungus Paecilomyces variotii Bainier with antimicrobial activity against Enterococcus faecalis ..................................................519 Serum as an environment to live or not to live for Gram-negative bacteria: relationship between lysozyme and complement system in killing Salmonella O48 strain ....................................................................................................................................523 Towards the eradication of Poliomyelitis............................................................................528 Methods – Quantitative Models and Bioinformatics in Microbiology ..................533 A brief note about the effect of microbial growth rate on the assimilation of toluene by Acinetobacter sp. ............................................................................................................535 Application of artificial neural networks to predict ochratoxin A accumulation in carbendazim-treated grape-based cultures of Aspergillus carbonarius...............................540 Evaluation of a Prototype Lateral Flow Device: Serological Test Kit for Rapid Detection of Potato Ring Rot Disease .................................................................................545 Expression, purification and characterization of the precursor of human pulmonary surfactant protein B (preproSPB) produced in Escherichia coli .........................................552 Microbial diversity, comparative analysis, and the use of molecular methods in natural environments ...........................................................................................................558 Microbial Physiology, Metabolism and Gene Expression ......................................563 Analysis of stability by elements at the proximal 3´-UTR of two KlCYC1 mRNAs ..........565 Characterization of Chitinase Gene from a Paca River Bacterium Chromobacterium violaceum UCP1489............................................................................................................569 xiii Characterization of KlHIS4 transcriptional regulation by growth-media nutrients.............574 Desulfovibrio vulgaris Hildenborough transcriptomic analysis by Restriction fragment functional display (RFFD)...................................................................................................577 Effect of some redox-mediators on the decolorization of Acid Orange 7 by resting Rhodococcus erythropolis and Alcaligenes faecalis cells ...................................................581 Heterologous expression and enzymatic characterisation of exopolygalacturonase PGX1 ...................................................................................................................................584 In silico and in vitro analysis of promoter regions of two exopolygalacturonase coding genes of Fusarium oxysporum . radicis lycopersici and regulation in Saccharomyces cerevisiae ...................................................................................................589 Modification by transposition in the bacterial production of poly-β-hydroxybutyrate (PHB) in Azospirillum brasilense.......................................................................................594 Preparation and characterization of Taxol loaded magnetic polymeric nanospheres..........597 Processing of stalled replication fork under thymine starvation and its relationship with thymineless death in Escherichia coli .........................................................................602 Targeting of exogenous β-carotene oxygenase into the chloroplast is essential for its efficient function in the microalga Chlamydomonas reinhardtii.........................................607 The analysis on different SigB concentration in Staphylococcus aureus ~High SigB accumulation enhances biofilm formation~ ........................................................................611 Two new members of the Tetrahymena multi-stress-inducible metallothionein family: T. rostrata Cd/Cu metallothioneins.....................................................................................615 Microbiology Education.............................................................................................619 A new strategy for introducing Secondary school students to Microbiology and Biotechnology......................................................................................................................621 The Microorganisms in the Portuguese National Curriculum and Primary School Textbooks ............................................................................................................................625 Yeast stress enzymes – application of microbiology and bioinformatics for initiate high school students in environmental studies ....................................................................630 Bioremediation............................................................................................................635 Changes in microbial population affected by physico-chemical conditions of soils contaminated by explosives.................................................................................................637 DNA and cDNA fingerprinting of 16S rRNA gene to assess the key organisms in low temperature methanogenic consortium................................................................................641 Effect of Dibenzothiophene on the Growth, on the Morphology and on the Ultrastructure of Cunninghamella elegans..........................................................................645 Evaluation of biosorption of Lead by Halomonas eurihalina strain NA-2 .........................651 xiv Lactic acid bacteria: a potential tool to reduce ochratoxin A in wine .................................656 The Real-Time Behavior of Chromium in Arthrobacter oxydans.......................................660 The role of Beta-Proteobacteria in aromatic hydrocarbon degradation: fingerprinting of 16S rRNA gene and catechol 2,3-dioxygenase gene by T-RFLP in BTEX degradative bacterial communities ......................................................................................664 Biosurfactants: Purification, Mass Production, Applications................................669 Applications of Surface Active Compounds by Gordonia in bioremediation and washing of hydrocarbon-contaminated soils .......................................................................671 Biosurfactant production by Chromobacterium prodigiosum.............................................676 Emulsifiers agents produced by Candida lipolytica cultivated in insoluble substrates.......681 Isolation and screening of surface active compound-producing bacteria on renewable substrates .............................................................................................................................686 Production of a bioemulsifier by Candida glabrata isolated from mangrove.....................691 Strategies of Optimization of Bioemulsifier Production by Candida lipolytica Using Semidefined Medium ..........................................................................................................696 Surface active agent produced by Candida lipolytica using cassava flour wastewater as substrate...........................................................................................................................701 Biotechnologically Relevant Enzymes and Proteins................................................707 Expression of different recombinant forms of the precursor of human pulmonary surfactant protein B (pro SP-B) in Pichia pastoris..............................................................709 Partial purification of xylose reductase from Candida guilliermondii for the use of the conversion of xylose into xylitol .........................................................................................715 Production of recombinant forms of the propeptide COOH-terminal and the saposin B-type domain of the propeptide NH2-terminal of the precursor of pulmonary surfactant protein B .............................................................................................................720 Protease enzyme for surface degradation of wool fiber to improve dyeability ...................725 Subcellular forms of cholesterol oxidase from Rhodococcus sp. CIP 105 335: induction, solubilization and characterization .....................................................................729 Microfactories – Microbial Production of Chemicals and Pharmaceuticals........737 Challenges attending upon studies on clavulanic acid production ......................................739 Growth and production of chitin and chitosan by Syncephalastrum racemosum using different carbon and nitrogen sources .................................................................................744 Improving the carbon conversion rate in Lactococcus lactis fermentations: Cloning strategies..............................................................................................................................749 xv Preparation of clavulanate salt using a tertiary octylamine as an intermediate ...................754 Recovery of clavulanic acid from an aqueous two-phase system by ion-exchange resin ...760 Study of Fouling Index in Tangential Filtration Applied for Separation of Clavulanic Acid from Fermentation Broths...........................................................................................765