Bacteria Culture
Implant caused infections are considered to be serious and common
complications in implant surgeries, and the problem usually requires removal
of prosthesis. This is a common concern for cardiovascular and orthopaedic
implants. Although success of orthopaedic and dental implants is mostly
dependent on the bone-implant osseointegration, the success and long term
survival of these implants are also dependent on the presence of the bacteria
surrounding the implants. To overcome these problems of bacterial infections,
it is vital that the materials which are to be implanted be analyzed for
bactericidal properties. After the biocompatibility test materials are therefore
required to be tested for bacterial response. In general (unless specified) a very
good antimicrobial properties are required. Thus bacteria culture tests are
performed on the samples to assess bactericidal properties.
Bacteria culture test isperformed by following a protocol. Sintered material is
initially polished and roughness of the surface is measured. Bacterial adhesion
is enhanced by the roughness of the material surface. So it is critical to
evaluate the roughness before doing bacteria culture. The roughness can be
evaluated using laser surface profilometer. As a part of sample preparation a
material is to be sterilized in a similar manner any orthopaedic implant or
surgical instruments are sterilized prior to the medical surgery. The
sterilization depends on the type of material, for metals or ceramics the
sterilization is done using steam autoclave while many polymers are sterilized
using ultraviolet exposure. After sterilization the sample are ultrasonicated for
20 minutes and placed in 4 well plate or 24 well plate depending on number of
samples. For any bacteria culture experiment one must use the control sample
which should exhibit known behaviour in terms of bactericidal behaviour. The
control sample is the thin glass disc. Now the samples are rinsed in 1X PBS
Implant caused infections are considered to be serious and common
complications in implant surgeries, and the problem usually requires removal
of prosthesis. This is a common concern for cardiovascular and orthopaedic
implants. Although success of orthopaedic and dental implants is mostly
dependent on the bone-implant osseointegration, the success and long term
survival of these implants are also dependent on the presence of the bacteria
surrounding the implants. To overcome these problems of bacterial infections,
it is vital that the materials which are to be implanted be analyzed for
bactericidal properties. After the biocompatibility test materials are therefore
required to be tested for bacterial response. In general (unless specified) a very
good antimicrobial properties are required. Thus bacteria culture tests are
performed on the samples to assess bactericidal properties.
Bacteria culture test isperformed by following a protocol. Sintered material is
initially polished and roughness of the surface is measured. Bacterial adhesion
is enhanced by the roughness of the material surface. So it is critical to
evaluate the roughness before doing bacteria culture. The roughness can be
evaluated using laser surface profilometer. As a part of sample preparation a
material is to be sterilized in a similar manner any orthopaedic implant or
surgical instruments are sterilized prior to the medical surgery. The
sterilization depends on the type of material, for metals or ceramics the
sterilization is done using steam autoclave while many polymers are sterilized
using ultraviolet exposure. After sterilization the sample are ultrasonicated for
20 minutes and placed in 4 well plate or 24 well plate depending on number of
samples. For any bacteria culture experiment one must use the control sample
which should exhibit known behaviour in terms of bactericidal behaviour. The
control sample is the thin glass disc. Now the samples are rinsed in 1X PBS
