(MB) ASCP Practice Exam Questions
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - Answer Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - Answer Thymine
What genes would be screened in a breast cancer panel? - Answer HER2, ERBB2,
BRCA1
Next Generation Sequencing uses: - Answer Short sequence reads
Purines and pyrimidines differ from each other in that: - Answer Purines have two rings;
pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - Answer Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - Answer 1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not
depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - Answer Nucleotide sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - Answer Initiation, exponential,
plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
,5'-GAC
3'-GAC
3'-CTG
5'-GTC - Answer 3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR indicates that patient has
Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - Answer 85 CAG repeats in the Huntington
gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - Answer 16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - Answer Helicase
Mutation in what gene is associated with Fragile X syndrome? - Answer FMR1
Mantle cell lymphoma (MCL) is caused by what translocation? - Answer t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity": -
Answer Pol α
Its discovery shed light on why there is simultaneous, though not continuous, synthesis
of DNA on both leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - Answer Okazaki fragments
What gene is measured following treatment with imatinib (Gleevec)?
, FLT3
BCR/Abl
Jak2
MAPK - Answer BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - Answer 50 nucleotides per second
This polymerase is involved in "replicates mitochondrial DNA": - Answer Pol γ
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods would best accomplish this
task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - Answer Standard PCR
Which of the following storage options is optimal for storing isolated DNA for a period
greater than seven years?
22-25ºC
2-8ºC
-20ºC
-70ºC - Answer -70ºC
Consider a hypothetical mutation involving gene X. Let's say you amplify a specific
exon, say exon 11, of that gene then you cut it with restriction enzyme W. In a person
without the mutation, cutting the gene with restriction enzyme W generates two
fragments of sizes, 100 bp and 250 bp. Suppose a C>T mutation in gene X deletes a
restriction site, yielding a fragment of 350 bp. You would expect a heterozygous person
for gene X to have these fragments on a restriction gel:
+/+ = 350 bp; 250 bp; 100 bp;
m/+ = Only the 350 bp
m/+ = 350 bp; 250 bp; 100 bp
m/m = 350 bp; 250 bp - Answer m/+ = 350 bp; 250 bp; 100 bp
Which of the following will more likely lower stringency conditions in the washing step of
a hybridization experiment?
Increase the concentration of salt in the wash solution buffer
Increase the temperature from, say 68°C to 75°C
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base - Answer Anti-codon
According to Chargaff's rule of base pairing, adenine pairs with: - Answer Thymine
What genes would be screened in a breast cancer panel? - Answer HER2, ERBB2,
BRCA1
Next Generation Sequencing uses: - Answer Short sequence reads
Purines and pyrimidines differ from each other in that: - Answer Purines have two rings;
pyrimidines have one ring
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine - Answer Adenine and guanine
What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs - Answer 1 in 1,000,000,000 base pairs
The rate of DNA migration through an agarose gel during electrophoresis does not
depend on which of the following factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule - Answer Nucleotide sequence of the molecule
What are the phases in a qPCR Amplification Plot?
Initiation, exponential, plateau
Baseline, exponential, plateau
Baseline, threshold, exponential, plateau
Baseline, initiation, threshold, exponential, plateau - Answer Initiation, exponential,
plateau
Find the palindrome in this restriction enzyme site: 5'-CTGCAG-3'?
,5'-GAC
3'-GAC
3'-CTG
5'-GTC - Answer 3'-GAC
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntington gene. Testing with standard PCR indicates that patient has
Huntington Disease, HD. Which of the following would be consistent with this
diagnosis?
25 CAG repeats in the Huntington gene
85 CAG repeats in the Huntington gene
25 CGA repeats in the Huntington gene
85 CGA repeats in the Huntington gene - Answer 85 CAG repeats in the Huntington
gene
Which two HPV types are responsible for most cases of cervical cancer?
16 and 18
31 and 59
16 and 58
44 and 59 - Answer 16 and 18
Replication forks, known as origins of DNA replication, are created by this enzyme:
Ligase
Taq Polymerase
Primase
Helicase - Answer Helicase
Mutation in what gene is associated with Fragile X syndrome? - Answer FMR1
Mantle cell lymphoma (MCL) is caused by what translocation? - Answer t(11;14)
This polymerase is involved in "initiation of DNA replication and has primase activity": -
Answer Pol α
Its discovery shed light on why there is simultaneous, though not continuous, synthesis
of DNA on both leading and lagging strands of DNA:
Klenow fragment of DNA polymerase
Okazaki fragments
Sanger fragments
RNA fragments - Answer Okazaki fragments
What gene is measured following treatment with imatinib (Gleevec)?
, FLT3
BCR/Abl
Jak2
MAPK - Answer BCR/Abl
What is the rate of mammalian DNA replication?
500 nucleotides per second
100 nucleotides per second
50 nucleotides per second
10 nucleotides per second - Answer 50 nucleotides per second
This polymerase is involved in "replicates mitochondrial DNA": - Answer Pol γ
A patient with impaired judgment, personality changes, signs of abnormal body
movements and depression comes to the physician's office for a follow-up visit. The
physician suspects a single-gene disorder may be the cause of those clinical
manifestations. A blood specimen was then sent to your clinical laboratory for mutation
screening in the Huntingtin gene. Which of these methods would best accomplish this
task?
Methylation-specific PCR
Standard PCR
PFGE
RAPD PCR - Answer Standard PCR
Which of the following storage options is optimal for storing isolated DNA for a period
greater than seven years?
22-25ºC
2-8ºC
-20ºC
-70ºC - Answer -70ºC
Consider a hypothetical mutation involving gene X. Let's say you amplify a specific
exon, say exon 11, of that gene then you cut it with restriction enzyme W. In a person
without the mutation, cutting the gene with restriction enzyme W generates two
fragments of sizes, 100 bp and 250 bp. Suppose a C>T mutation in gene X deletes a
restriction site, yielding a fragment of 350 bp. You would expect a heterozygous person
for gene X to have these fragments on a restriction gel:
+/+ = 350 bp; 250 bp; 100 bp;
m/+ = Only the 350 bp
m/+ = 350 bp; 250 bp; 100 bp
m/m = 350 bp; 250 bp - Answer m/+ = 350 bp; 250 bp; 100 bp
Which of the following will more likely lower stringency conditions in the washing step of
a hybridization experiment?
Increase the concentration of salt in the wash solution buffer
Increase the temperature from, say 68°C to 75°C
