Unit 2 Learning Aim C Distinction Acheived

Unit 2




Unit 2 Learning aim C

(Name)

(School)

Applied Science




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, Unit 2

Unit 2 Learning aim C

Chromatography of leaves: (paper)

Equipment:

Pestle and mortar

Chromatography paper

Teat pipette

100cm3 beaker

Small capillary tube

Sand

Leaves and scissors

Propanone

Preparation of plant solution:

When making the solution, it was critical to keep the concentration high while keeping the

volume low, ensuring that the pigment was not overly diluted. The separation would have been

impossible to notice if the solution had been too diluted.

To prepare the plant solution, the leaves were first cut into little pieces, making it easier to crush

the leaves. The leaves were then broken down using sand and a little amount of propanone.

Using a pestle and mortar, the leaves were mashed to create a small amount of concentrated

colour solution.

Method for paper chromatography:

The liquid from the leaf extract was applied to the centre of the line with a fine glass tube, and

the area was then allowed to dry. This was done several times to get a highly concentrated tiny

spot of chromatography paper.



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