“Lab 5 Microbial Nutrition” “BIO250L”
Student Name:
Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Bio-Prospecting for Starch Degrading Bacteria”
“Table 3: Gram Iodine Analysis”
“Sample” “Growth on Starch Agar “Clear Area Around “Is Sample Capable of
(yes or no)” Bacteria?” Degrading Starch?”
“A” Yes. Yes Yes
“B” Yes Yes Yes
“C” Yes Yes Yes
“D” No Yes No
“Post-Lab Questions”
“1. Why was cow manure used as a potential source of starch degrading bacteria?”
Because it contains amylase which is an enzyme that breaks down starch into smaller molecules. Cow
manure is used because of the high starch content and amylase which is responsible for degrading
starch.
“2. What are some other potential sources for starch degrading bacteria?”
Starch degrading bacteria are mainly important for industries that have fermentation, textile, paper and
also food.
“3. What component makes the starch agar selective for amylase degrading bacteria?”
Starch agar is a medium that tests the ability for an organism to produce a certain exoenzymes, which
includes amylase and others that hydrolyze the starch.
“4. Why were each of the following steps performed in this experiment?”
“a. Serial dilution”
It was helpful in obtaining an appropriate number of cells for the assessment. It also reduces density of
the cultures in the cells.
“b. Growth on the nutrient agar plates”
Student Name:
Access Code (located on the lid of your lab kit): Click here to enter text.
“Experiment 1- Bio-Prospecting for Starch Degrading Bacteria”
“Table 3: Gram Iodine Analysis”
“Sample” “Growth on Starch Agar “Clear Area Around “Is Sample Capable of
(yes or no)” Bacteria?” Degrading Starch?”
“A” Yes. Yes Yes
“B” Yes Yes Yes
“C” Yes Yes Yes
“D” No Yes No
“Post-Lab Questions”
“1. Why was cow manure used as a potential source of starch degrading bacteria?”
Because it contains amylase which is an enzyme that breaks down starch into smaller molecules. Cow
manure is used because of the high starch content and amylase which is responsible for degrading
starch.
“2. What are some other potential sources for starch degrading bacteria?”
Starch degrading bacteria are mainly important for industries that have fermentation, textile, paper and
also food.
“3. What component makes the starch agar selective for amylase degrading bacteria?”
Starch agar is a medium that tests the ability for an organism to produce a certain exoenzymes, which
includes amylase and others that hydrolyze the starch.
“4. Why were each of the following steps performed in this experiment?”
“a. Serial dilution”
It was helpful in obtaining an appropriate number of cells for the assessment. It also reduces density of
the cultures in the cells.
“b. Growth on the nutrient agar plates”
