BIOD 171/Microbiology Lab 9 Exam Portage Learning

BIOD 171/Microbiology Lab 9 Exam Portage Le



Question 1


Using the observations made and recorded from PL09 Unknown Pathogen, compl
sections accordingly.



Given Culture A and Culture B were accidentally mixed, what observations we
the Gram stain
(shown below)?




Your Answer:
1. Gram stain => Pink rods/clusters- negative - Pseudomonas or E. Coli / Purple
positive
- Staph aureus or Strep




Gram-positive cocci (spherical) clusters and Gram-negative bacillus (rods) wer




Question

Using the observations made and recorded from PL09 Unknown Pathogen, compl

, Your Answer:
Culture A: no growth
Culture B: red color - only gram negative will grow
Used as a selective media given that only gram-neg bacteria will grow. Cult




MacConkey agar is selective for Gram-negative bacteria. Thus, Culture A mus
Culture B must be Gram-negative (growth observed) and this correlates to th




Question

Using the observations made and recorded from PL09 Unknown Pathogen, compl
sections accordingly.



The hemolytic properties of both cultures were next examined by streaking e
on a blood agar plate (shown below). What is the hemolytic status of Culture