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Samenvatting Ontwikkelingsproces Zebravis Embryologie En Teratologie (1047FBDDIE)

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Beschrijving van het ontwikkelingsproces van de zebravis in stappen. Telkens met de belangrijkste kenmerken en foto's opgelijst.

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EMBRYONALE ONTWIKKELING ZEBRAVIS

ZYGOTE PERIODE
0-0.75 hpf
- Vanaf bevruchting tot 45 min erna
- Zygote tot 1-cellig stadium
- Vegetable en animalpole




KLIEVINGPERIODE 1
0.75-2.25 hpf
- Klieving cytoplasma
- Gn volledige delingen (nog CP bruggen)
- 1ste 5 delingen loodrecht op elkaar
- Vorming blastomeren
- Vorming blastodisk




BLASTULAPERIODE 1+2
2.25 – 5.25 hpf
- Vorming van (einde 10de celdeling)
o Yolk syncytial layer (YSL), door
blastodiskcellen dichtst bij CP
o Enveloping layer (EVL)
o Deep cell layer (DEL)
o Blastodisc = EVL + DEL




BLASTULAPERIODE 3
- Blastodisk krijgt sferische vorm




BLASTULAPERIODE 4
- Start epiboly

, BLASTULAPERIODE % EPIBOLY
- Blastodisk  blastoderm
- 30% v/d dooier door blastoderm bedekt
- Einde van de blastulaperiode




GASTRULAPERIODE 1
5.25 – 10 hpf
- epiboly gaat verder in verschillende stappen
GASTRULAPERIODE 2+3+4/ 50% EPIBOLY
- Stoppen op 50% en even op dit niveau blijven
- Fate map of DEL
o Ontwikkeling kiembladen in DEL
- Vorming van de GERM RING (kiem ring)
o Germ ring = EVL + epiblast + hypoblast
o EVL  periderm
o Epiblast  ectoderm
o Hypoblast  mesoderm + endoderm
- Vorming embryonic shield
o Embryonic shield = locale verdikking
epiblast en hypoblast (=DEL)
o Komt overeen met de dorsale zijde v/h
embryo
o °DV-as en AP-as (onderscheiden rug/buik
regio en kop/staart regio)


GASTRULAPERIODE 5/ 75% EPIBOLY
- DEL-cellen in shield verspreiden zich over AP-as
- Hypoblast
 Axiaal  notochord + endoderm
 Paraxiaal  spieren kopregio + somieten




GASTRULAPERIODE 6/ 90% EPÏBOLY
- Overblijfsel dooier (yolk plug)
- Start hersenaanleg
 Verdikking anterieure epiblast
 °Neurale plaat
 °hersenaanleg




GASTRULAPERIODE 7/ 100% EPIBOLY
- Einde van de gastrula
- Staartknop
- Neurale plaat langst hele embryonale axis

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