Summary Histology Introduction-Lesson 1

Histology Clearing- making your
tissue translucent
Significance to Medicine:
Reagent: Xylene
-Histology Technicians (in other countries) deals Infiltration -fill in holes with
with tissue that are analysed in laboratory Reagent: melted paraffin
(Biopsy) wax
Embedding- make tissue
-Pathologists- abnormality of tissues (body) block with
caused by a disease. – Head, assisted by Med Reagent: paraffin wax
tech. Trimming
Terms: Sectioning- with microtome
Product: tissue ribbons
 Histology- “histo” + “logos”- study of Then, put tissue ribbon in
microanatomy of cells and tissue water bath 60oC.
formation, structure, and functions) Meyers Albumin- for gluing
 Pathology- “pathos”+ “logos”- study the slide
and diagnosis of diseases through Staining:
microscopic examination of surgically  H stain
removed organs, tissues (biopsy - Haematoxylin (Purple)
samples), bodily fluids, and in some - Basic dye
cases the whole body (autopsy). - Take up by nucleus (acid
 Histopathology- diagnosis of tissue that components of cell)
altered by disease  E stain
Organization in the body - Eosin (Orange)
- Acid dye
 atoms, molecules, organelles, - Take up by cytoplasm (basic
cells, tissues, organs, organ component of cell)
systems, and the human Mounting- prevent scratches
organism. Tissue with colour- add adhesive
Tissue section: (Canada Balsam (clear nail polish))
Label
 Longitudinal - ( I )
 Cross/ Transverse- ( - )
 Oblique- ( / )

Tissue preparation:

Fixation- ex: Tissue sample from
tumor
Reagent: Formalin, 10%
formaldehyde solution

Dehydration-removal of water
Reagent: Increasing concentration
of alcohol from 50% to 100%