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Samenvatting Primaire kenmerken

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Bacteriële primaire kenmerken

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Uploaded on
January 15, 2022
Number of pages
3
Written in
2019/2020
Type
Summary

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Primaire kenmerken
1)Morfologische kenmerken
 Kleur
 Grootte
 Type (Rough, Smooth, Dwarf, Mucoid)

> Vorm, grootte, groepsveMurband
> Gram+ , Gram- , zuurvast
> sporen (vooral in oude culturen) + ligging ervan



2)Groeivoorwaarden
Omgevingsatmosfeer Temperatuurtolerantie
 Aëroob  De meeste bacteriën groeien bij 36°C
 Anaeroob  Campylobacter bij 42°C
 Verhoogde CO2 spanning  Yersinia bij 24°C

> dimorfe fungi: mucoïde gistvorm (36°C), pluizige
schimmel (25°C)
Zouttolerantie Galtolerantie en galstimulatie
 Halofiel = groeien bij hoge  Bacteriën in de darm moeten in staat
zoutconcentratie zijn om te overleven in aanwezigheid
van gal en galzouten
> Vb: Vibrio
> zouttolerante bacteriën kunnen zelf concentraties > galzouten worden gebruikt in voedingsbodems
van 6,5% NaCl verdragen (S. aureus en (McC en HEA)
enterococcen) > Bacteroides fragilis wordt zelfs in groei
gestimuleerd bij aanwezigheid van gal



3)Gevoeligheid voor chemische stoffen en antibiotica
 Kan een geïsoleerde cultuur overleven in de aanwezigheid van een bepaalde groei
inhiberende stof?

> Antibiotica schijfjes: bacitracine, novobiocine, colistine of erythromycin. (= veel gebruikt)



4)Pigmentproductie
 Sommige bacteriën produceren een pigment en dat onder bepaalde omstandigheden
 Samenstelling voedingsbodem, temperatuur, licht, enz…

> Pseudomonas aeruginosa: pyocyanine = blauw/groen
> Serratia marcescens: rood pigment bij 30°C




5)Hemolyse
 α −h emolyse = Hb > CAMP-test Omgekeerde CAMP-test
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