Class notes Life Sciences (bio1) Biochemistry, 5th Edition (Updated and Revised Edition)-E-Book, ISBN: 9788131262542

BIOCHEMICAL TECHNIQUES

1. In gel filteration, biomolecules are separated
according to:
a) The net charge
b) The difference in molecular weight
c) Ability of diffusion through porous membranes
d) Polarity
e) Solubility

2. Radio Immune Assay (RIA):
a) Is used for purification of hormones
b) Involves the use of radioactive elements
c) Is used for quantitative measurements of
hormones
d) Requires a pure antigen of known
concentration
e) "A" is the only wrong statement

3. Electrophoresis:
a) Involves the use of polyacrylamide gel
b) Can be used for purification of proteins and
nucleic acids
c) Is usually followed by Western blotting for
proteins
d) A, B and C are all correct
e) Only B is correct

4. The following technique is the most suitable for the
separation of a mixture of the 20 amino acids:
a) Two dimensional electrophoresis
b) Gel filteration
c) Affinity chromatography

, d) Dialysis
e) RIA

5. Chromatography:
a) Is a technique for protein sequencing
b) Is a separation technique
c) Involves a mobile phase and stationary phase
d) Is exemplified by gel filteration
e) Only B,C and D are correct

6. Dialysis:
a) Involves separation of molecules according to
density
b) Is based on diffusion across a porous
membrane
c) Is a type of chromatography
d) Can be used to separate 2 molecules with the
same molecular weight
e) The molecular weight is not important in the
technique




7. Ion exchange chromatography:
a) Used for sequencing of proteins
b) Useful in separation of proteins
c) Affinity chromatography
d) Dialysis
e) RIA