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Summary Food Microbiology (FHM20306) Pt.2

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Part 2 of the complete summary of the course material necessary for the exam of food microbiology

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October 4, 2021
Number of pages
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Written in
2021/2022
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Hygiene and control

Control of food safety
How to assure food safety?

best strategies

1. regular cleaning
2. control critical steps during processing

Safeguard product during processing by;

- correct processing
- prevent contamination
- working correctly

end-product testing

- not the best method
- expensive and results often come too late when the product has already reached its expiry
date/has been consumed.

Cleaning: why and how?
Def: the removal or soil, food residue, dirt, grease or other objectionable matter

- does not mention microorganisms?
- but dirt does have an effect on the microorganisms present...

Effects of food residues

- acts as a source of nutrients
- physically protects microorganisms
- chemically reduces the effect of disinfectants because disinfectant then react with the dirt
instead of the microorganisms
- decreases the performance of equipment (eg heat exchangers)

Cleaning vs disinfection

, - Organic soils are most relevant in the food industry
- after determining the nature of the soil, you can determine the best cleaning method

3 aspects of cleaning




- CIP = cleaning in place

Water

- hard water (containing ions Ca2+ and Mg+) can result in scale formation
- acid can be used to clean this but is not advised for everyday cleaning/frequently used
household appliances (kettle)

Alkali/caustic

- used with stainless steel, rubber and synthetic materials
- corrosive: aluminum, tin
- harmful to skin, not done by hand

Detergents

- helps to dissolve water-insoluble compounds
- detergents have polar head and apolar tails and form micelles
- helps to wet the surface

Chemical: sequestering/chelating agents (found in detergents)

- keeps salt in solutions
- prevents deposits
- aids in emulsifying
- may add buffering + wetting

protective colloids

- set viscosity

, Hygienic design
Hygienic equipment = equipment that can be cleaned and freed from relevant microorganisms

- class I: in-place, without dismantling
- class II: after dismantling

Aseptic equipment: hygienically designed equipment that is sterilizable and is impermeable to
microorganisms during production which allows it to maintain its aseptic status.

Hygienic design = cleanability

Disinfection / sanitation
- disinfectant should reduce the number of microbes on a surface with 99.999% (5 log
reduction) in a standard test
- The surface should be CLEANED before disinfection

Methods of disinfection

1. heat (most common method)
- principle: denaturation of proteins
- dry heat / wet heat (steam)
- wet heat is more effective

2. UV radiation
- principle: damage to RNA and DNA
- can only be applied to clear liquids; sterilization of filtered water

3. chemicals
- disinfectants / sanitizers

Different type of chemical disinfectants

Oxidizers

- principle: oxidation, disturb protein synthesis and enzyme systems by removal of Sulphur
groups

cons:

- halogens (Cl, I) sensitive to soil (dirt)
- hydrogen peroxide: degraded by catalase
- peracetic acid: unstable, corrosive
- ozone: very unstable, corrosive

Alcohol

- principle: cause membrane leakage
- NOTE: higher concentrations are bacteriostatic as the organism is dehydrated and can
continue to grow once water is present again
- 60-70% are bactericidal, kills microorganisms (most effective)
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