BIOLOGICAL SCIENCE 2Ed-Canada by Scott Freeman Ch16-Transcription, RNA Processing, and Translation

1 DroneIT Biological Science, Canadian Edition., 2e (Freeman et al.) Chapter 16 Transcription, RNA Processing, and Translation Figure 16.1 1) The enzyme complex associated with DNA in the figure is A) helicase. B) DNA polymerase. C) RNA polymerase. D) topoi somerase. Answer: C Reference: Section 16.1 Bloom's Level: Remembering LOs: Chp16 -1. Relate the structure of RNA polymerase to its function in transcription. 2) RNA polymerase needs a subunit to initiate transcription that is not needed for transcript elongation. What is the subunit? A) Mg 2+ B) rho C) sigma D) the holoenzyme Answer: C Reference: Section 16.1 Bloom's Level: Remembering LOs: Chp16 -1. Relate the structure of RNA polymerase to its function in transcription. 2 DroneIT 3) The segments of DNA whe re transcription begins have a binding site for RNA polymerase. These segments are known as A) sigma. B) the holoenzyme. C) initiation factors. D) promoters. Answer: D Reference: Section 16.1 Bloom's Level: Remembering LOs: Chp16 -2. Describe the steps in initiation, elongation, and termination of transcription and translation. 4) David Pribnow studied the base sequences of promoters in bacteria and bacterial viruses. He found two conserved regions in these promoters (the -10 box and the -35 box). What is the function of these two regions of the promoter? A) They signal the initiation site. B) They bind the sigma subunit that is associated with RNA polymerase. C) They attach the correct nucleotide triphosphate to the template DNA strand. D) They separate the two DNA strands. Answer: B Reference: Section 16.1 Bloom's Level: Remembering LOs: Chp16 -2. Describe the steps in initiation, elongation, and termination of transcription and translation. 5) Which of the following processes is central to the init iation of transcription? A) binding of sigma to the promoter region B) formation of a phosphodiester bond in the elongating RNA strand C) binding of DNA polymerase to the promoter region D) formation of a DNA primer Answer: A Reference: Section 16.2 Bloo m's Level: Remembering LOs: Chp16 -2. Describe the steps in initiation, elongation, and termination of transcription and translation. 3 DroneIT 6) Put the following events of transcription in chronological order. 1. Sigma binds to the promoter region. 2. The do uble helix of DNA is unwound, breaking hydrogen bonds between complementary strands. 3. Sigma binds to RNA polymerase. 4. Sigma is released. 5. Transcription begins. A) 2, 3, 4, 5, 1 B) 2, 3, 1, 4, 5 C) 3, 1, 2, 5, 4 D) 3, 2, 1, 4, 5 Answer: C Refere nce: Section 16.1 Bloom's Level: Remembering LOs: Chp16 -2. Describe the steps in initiation, elongation, and termination of transcription and translation. 7) What is the function of the group of amino acids on the RNA polymerase called the rudder ? A) It provides the energy required for formation of phosphodiester bonds in the elongation RNA molecule. B) It helps unwind and open the DNA molecule. C) It moves template and non -template strands of DNA through channels inside the enzyme. D) It helps sigma bi nd to the RNA polymerase molecule. Answer: C Reference: Section 16.1 Bloom's Level: Remembering LOs: Chp16 -1. Relate the structure of RNA polymerase to its function in transcription. 8) During elongation, RNA polymerase has three prominent channels, or grooves. These channels provide sites for all of the following, EXCEPT A) a site for the double -stranded DNA molecule. B) a site for the entry of ribonucleoside triphosphates. C) a site for the exit of the diphosphates removed from the nucleotide triphos phates. D) a site for the growing RNA strand. Answer: C Reference: Section 16.1 Bloom's Level: Remembering LOs: Chp16 -2. Describe the steps in initiation, elongation, and termination of transcription and translation.