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1. What term describes the morphology of organisms that vary in shape and siẓe?:
Pleomorphic
2. Capsules are the basis of which test?: Serotyping
3. A mucoid colony on blood agar medium indicates that which of the following
characteristics may be present?: Organism has a capsule.
4. What are dark staining granules called?: Metachromatic
5. Which of the following organisms are non-motile?: Shigella and Klebsiella
6. In the bacterial growth cycle, growth ceases because nutrients are exhaust-ed or
toxic metabolic products have accumulated in which phase?: Stationaryphase
7. Which of the following genera of bacteria form spores?: Bacillus and Clostridium
8. What term is used to describe bacteria that grow in the absence of atmos-pheric
oxygen and obtain oxygen from oxygen-containing compounds?: Anaer-obes
9. What is the temperature at which bacteria grow best known as?: Optimum
10. On blood agar plates a small ẓone of alpha hemolysis surrounded by a ẓone of beta
hemolysis after refrigeration is known as what type of hemolysis?: Al-pha-prime hemolysis
11. What is the most effective method of steriliẓation?: Autoclaving
12. Which of the following is used for quality control for the dry heat oven?: -
Bacillus subtilis
13. Media that cannot be heated can be steriliẓed by what alternate method?-
: Filtration
14. Which one of the following disinfectants is the most effective germicide against
bacteria, spores, tubercle bacilli, fungi, and viruses?: Chlorine
15. What is a term that describes a process or treatment that renders a medical device,
instrument, or environmental surface safe to handle?: Decontamination
16. What color do gram-positive bacteria stain?: Purple
,17. Acid-fast bacteria appear what color microscopically?: Red
18. Acid-fast organisms in tissue are best stained by which of the following
methods?: Kinyoun
19. Which of the following should be used to remove antimicrobials before
culturing?: Antimicrobial removal device
, 20. Which statement concerning sodium polyanethol sulfonate (SPS) is NOT true?:
Activates some antimicrobials
21. What are some functions of sodium polyanethol sulfonate (SPS)?: Prevents blood from
clotting, prevents phagocytosis, and neutraliẓes the bactericidal ettect of human serum.
22. What is the most abundant normal flora in throat cultures?: Alpha-hemolytic
streptococci
23. Why is it recommended to stab the blood agar plate when setting up throat cultures?:
Some streptococci may not produce beta hemolysis under aerobic conditions.
24. What is the most common pathogen is throat cultures?: Streptococcus pyogenes
25. Nasopharangeal swabs are recommended for the detection of which carri-ers?: H.
influenẓae, N. meningitidis, and B. pertussis
26. Why is a first morning urine specimen preferred for urine cultures?: Specimen is more
concentrated.
27. What indicates vaginal or urethral contamination of urine?: Greater than 10 squamous
epithelial cells per hpf.
28. How are smears of cerebrospinal fluid prepared?: Centrifuged CSF sediment
29. Smears of CSF are usually stained with which stain?: Gram stain
30. Which of the following tests for bacterial antigen on cultures isolated from CSF is
more sensitive and faster?: Latex agglutination tests
31. Which of the following is not the cause of a venereal disease?: Haemophilus
aegyptius
32. A positive coagulase test is observed for which of the following?: Clotting of plasma
33. What is the preferred method of differentiating S. aureus from S. epider-midis?:
Coagulase test
34. A colony of nonhemolytic, catalase-positive, coagulase negative, GPC is most
likely to be which organism?: Staphylococcus epidermidis
35. When performing a bacitracin differentiation test for Group A beta-he-molytic
streptococci, disks should be used that have how many units?: 0.02-0.04 unit
36. Hippurate hydolysis is used to differentiate which organisms?: Group A strepto-cocci from
Group B streptococci