MICROBIOLOGY UNE LECTURE FINAL PRACTICE
QUESTIONS AND CORRECT ANSWERS
For each microbe, designate the level of resistance to antimicrobial control using the
terms provided:
- Protozoan cyst, fungi
- Enveloped virus
- Endospore
- Prion
- Vegetative bacteria - Answers - - Medium resistance
- Lowest resistance
- Highest resistance
- Medium resistance
- Medium resistance
You want to examine the surface structure of a virus outer protein coat by microscopy.
Which of the following would be the microscope of choice and why? - Answers - An
atomic force microscope has the highest resolution and magnification of the currently
available microscopes.
Which 2 structures play direct roles in permitting bacteria to adhere to each other or
other surfaces? - Answers - Capsules
Fimbriae
Which control method would not be suitable choice for killing Mycobacteria in a capped
culture tube? - Answers - UV (germicidal light)
Prokaryotic and eukaryotic cells generally have which of the following features in
common? - Answers - Ribosomes
Placing organisms at 4 C is: - Answers - Bacteriostatic
Your supervisor asks you to set up a pure culture of a bacterial species he is
researching. He tells you that this species is a fastidious, halophilic, gram-positive
mesophile that doesn't have catalase or superoxide dismutase. What type of culture
medium and environmental factors must you use to grow this organism? - Answers -
Blood agar, 25% NaCl, 35C, in an anaerobic chamber
Glycolysis and the pentose phosphate pathway both produce: - Answers - pyruvate
Denaturation - Answers - the action of heat or chemicals on proteins whose function
depends on an unaltered tertiary state
, Alcoholic fermentation - Answers - Extraction of energy through an incomplete,
anaerobic degradation of glucose into ethanol
Glycolysis - Answers - The energy-yielding breakdown of glucose to pyruvic acid; no
molecular oxygen is consumed in the degradation
Oxidative Phosphorylation - Answers - Synthesis of ATP using energy given off during
the electron transport phase of respiration
Catabolism - Answers - Chemical breakdown of complex compounds into simpler units
to be used in cell metabolism
All bacterial cells that result from the replication of a single original bacterial organism
are said to be a: - Answers - pure culture
Which of the following methods of enumerating cells is the only one to exclusively
detect live cells - Answers - Viable plate count
A lab tech has two pure isolated biological samples he is working with; one is a virus
and one is a viroid. The labels come off the tubes during transport. The tech is sure he
can distinguish between the two samples by analyzing for the presence of a single type
of molecule. Which type of molecule is he looking for? - Answers - DNA
Eucarya - Answers - Have more complex internal structures than archaea or bacteria
and have membrane around the DNA.
Which pair is mismatched?
Iodophor-iodine
Formalin-formaldehyde
Benzalkonium chloride-quarternary ammonium compound
Phenolics-peracetic acid
Sodium hypochlorite-chlorine - Answers - Phenolics-peracetic acid
Which is not true concerning the cell wall of bacteria? - Answers - it is the site of group
translocation
Which of the following statements is correct about gram-negative bacteria? - Answers -
They possess an outer membrane containing toxic LPS
Studies done by Buchner showed that ground-up yeast cells were able to convert sugar
to alcohol. The components of the mixture that were responsible for this transformation
were: - Answers - Enzymes
If the processes of aerobic and anaerobic respiration are basically similar - differing only
in the terminal electron acceptor - why do they produce different amounts of energy? -
Answers - They produce different amounts of energy because not all electron acceptors
QUESTIONS AND CORRECT ANSWERS
For each microbe, designate the level of resistance to antimicrobial control using the
terms provided:
- Protozoan cyst, fungi
- Enveloped virus
- Endospore
- Prion
- Vegetative bacteria - Answers - - Medium resistance
- Lowest resistance
- Highest resistance
- Medium resistance
- Medium resistance
You want to examine the surface structure of a virus outer protein coat by microscopy.
Which of the following would be the microscope of choice and why? - Answers - An
atomic force microscope has the highest resolution and magnification of the currently
available microscopes.
Which 2 structures play direct roles in permitting bacteria to adhere to each other or
other surfaces? - Answers - Capsules
Fimbriae
Which control method would not be suitable choice for killing Mycobacteria in a capped
culture tube? - Answers - UV (germicidal light)
Prokaryotic and eukaryotic cells generally have which of the following features in
common? - Answers - Ribosomes
Placing organisms at 4 C is: - Answers - Bacteriostatic
Your supervisor asks you to set up a pure culture of a bacterial species he is
researching. He tells you that this species is a fastidious, halophilic, gram-positive
mesophile that doesn't have catalase or superoxide dismutase. What type of culture
medium and environmental factors must you use to grow this organism? - Answers -
Blood agar, 25% NaCl, 35C, in an anaerobic chamber
Glycolysis and the pentose phosphate pathway both produce: - Answers - pyruvate
Denaturation - Answers - the action of heat or chemicals on proteins whose function
depends on an unaltered tertiary state
, Alcoholic fermentation - Answers - Extraction of energy through an incomplete,
anaerobic degradation of glucose into ethanol
Glycolysis - Answers - The energy-yielding breakdown of glucose to pyruvic acid; no
molecular oxygen is consumed in the degradation
Oxidative Phosphorylation - Answers - Synthesis of ATP using energy given off during
the electron transport phase of respiration
Catabolism - Answers - Chemical breakdown of complex compounds into simpler units
to be used in cell metabolism
All bacterial cells that result from the replication of a single original bacterial organism
are said to be a: - Answers - pure culture
Which of the following methods of enumerating cells is the only one to exclusively
detect live cells - Answers - Viable plate count
A lab tech has two pure isolated biological samples he is working with; one is a virus
and one is a viroid. The labels come off the tubes during transport. The tech is sure he
can distinguish between the two samples by analyzing for the presence of a single type
of molecule. Which type of molecule is he looking for? - Answers - DNA
Eucarya - Answers - Have more complex internal structures than archaea or bacteria
and have membrane around the DNA.
Which pair is mismatched?
Iodophor-iodine
Formalin-formaldehyde
Benzalkonium chloride-quarternary ammonium compound
Phenolics-peracetic acid
Sodium hypochlorite-chlorine - Answers - Phenolics-peracetic acid
Which is not true concerning the cell wall of bacteria? - Answers - it is the site of group
translocation
Which of the following statements is correct about gram-negative bacteria? - Answers -
They possess an outer membrane containing toxic LPS
Studies done by Buchner showed that ground-up yeast cells were able to convert sugar
to alcohol. The components of the mixture that were responsible for this transformation
were: - Answers - Enzymes
If the processes of aerobic and anaerobic respiration are basically similar - differing only
in the terminal electron acceptor - why do they produce different amounts of energy? -
Answers - They produce different amounts of energy because not all electron acceptors
