Biochem Exam 4
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, Explain the Generational Results of Messelson and Stahl Results confirm the semi-
Experiment by Density Gradient Centrifugation conservative replication of
DNA. One DNA strand
serves as a "template" for
replication of new daughter
strand, conserving half of
the original DNA molecule
in the second generation
DNA, etc.
What is DNA replication? Summerize the process of The process by which DNA is synthesized, creating an exact copy of itself.
DNA replication. During replication, the two strands of the DNA double helix separate (unzip),
and each original strand serves as a template for the synthesis of a new,
complementary strand. This involves complementary base pairing (where
adenine (A) pairs with thymine (T) and guanine (G) pairs with cytosine (C))
between the old parental strand and the newly synthesized strand. The result is
two DNA molecules, each consisting of one original strand and one new strand
(is semiconservative).
What is the function of helicase? Unzips the DNA
What is the function of SSBP? Single stranded binding proteins stabilize the single DNA strand by holding it
open (preventing reattachment)
What is the leading strand? The strand that is copied in the direction toward the replication fork and is
synthesized continuously
What is DNA polymerase 3? Synthesizes new strand by adding fragments to the primer and synthisises the
continueusly to its growing 3' end (toward the replicasoin fork)
What is primase? Enzyme that makes an RNA primer on which DNA polymerase III can initiate
replication.
What is a primer? RNA laid down for synthesis so that DNA polymerase 3 can build on it
What is ligase's function? Closes any nicks, breaks, or gaps on the lagging strand and seals Okazaki
fragments.