CELL BIO EXAM 1 UKY BIO 315 QUESTIONS & ANSWERS

CELL BIO EXAM 1 UKY BIO 315
QUESTIONS & ANSWERS



objective lens - Correct Answers -determines the degree of resolution that can be
achieved by the microscope

condenser - Correct Answers -used to alter the amount of contrast visible in the
specimen

wavelength, cone angle, refractive index - Correct Answers -all contribute to the
resolution of a microscope

histone-GFP expression - Correct Answers -method used to visualize the histone
component of chromosomes

Mouse IgG and Mouse 1 antibody - Correct Answers -proteins recognized by the 2
antibodies used in immunostaining

fluorescence microscopy of a GFP tagged protein - Correct Answers -method used to
monitor the dynamic behavior of a specific protein in a living cell

blue light and 1 NA - Correct Answers -best resolution

anti-histone 1 antibody - Correct Answers -provided the specificity needed to selectively
visualize histones in the nuclei of immunostained embryos

DAPI - Correct Answers -a fluorescent stain that can be used to specifically stain DNA

attachment via covalent linkage and injection - Correct Answers -methods used in vitro
to label a purified protein

SDH activity - Correct Answers -used to identify fractions containing mitochondira

DCIP - Correct Answers -provided a colorimetric indicator of the REDOX reaction in the
succinate assays

malonate - Correct Answers -acted as the inhibitor in the succinate assays