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Cham Lab Questions and Answers with Verified Solutions | Latest 2026 Update

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Cham Lab Questions and Answers with Verified Solutions | Latest 2026 Update

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Cham Lab Questions and Answers with Verified Solutions | Latest 2026
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Q: micro-volume spectrophotometer

Answer:
compress 1-2.5uL between 2 pedestals
compressed sample forms a short column and light is delivered and det3ected using
fiber
optical cable inserted into the center of both pedestals

Q: detectors

Answer:
quantify intensity and sometimes wavelength of light
convert light to electrical signal and amplify it
to send to computer

Q: PMT, CCD, silicon photodiodes

Answer:

, Cham Lab Questions and Answers with Verified Solutions | Latest 2026
Update




Q: if absorbance is 2

Answer:
how much light absorbed by analyte 99

Q: why do absorption spectra have different peaks, valleys, and flat regions

Answer:
because e changes
at every wavelength

Q: SHAPE of absorption spectra

Answer:
will not change if the concentration is different
only the height of regions will change proportionally with C

Q: why do we calibrate the spectrophotometer

Answer:
accounting for instrument error

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