,
, HIMA 100 LAB 6: QUANTITATION OF
CULTURED MICROORGANISMS LAB
ASSIGNMENT | 2026 UPDATE | 100%
CORRECT | WITH COMPLETE
SOLUTIONS.
SECTION 1: SERIAL DILUTION FUNDAMENTALS
(Questions 1-10)
Question 1
What is the purpose of performing a serial dilution in microbiology?
A) To kill all bacteria in the sample
B) To reduce the concentration of a sample to a countable range
C) To increase the number of bacteria for easier counting
D) To identify the species of bacteria present
Answer: B) To reduce the concentration of a sample to a countable
range
Rationale: Serial dilution is used to reduce the concentration of
microorganisms in a sample so that when plated, the resulting colonies are
countable . Because a sample may contain millions of cells per milliliter, it
must be diluted serially to achieve a plate with between 30 and 300
colonies . Samples with too many colonies are designated "Too Numerous To
Count" (TNTC), while those with fewer than 30 colonies are "Too Few To
Count" (TFTC) .
Section: Serial Dilution - Purpose
Source: HIMA 100 Lab 6 Assignment
, HIMA 100 LAB 6: QUANTITATION OF
CULTURED MICROORGANISMS LAB
ASSIGNMENT | 2026 UPDATE | 100%
CORRECT | WITH COMPLETE
SOLUTIONS.
SECTION 1: SERIAL DILUTION FUNDAMENTALS
(Questions 1-10)
Question 1
What is the purpose of performing a serial dilution in microbiology?
A) To kill all bacteria in the sample
B) To reduce the concentration of a sample to a countable range
C) To increase the number of bacteria for easier counting
D) To identify the species of bacteria present
Answer: B) To reduce the concentration of a sample to a countable
range
Rationale: Serial dilution is used to reduce the concentration of
microorganisms in a sample so that when plated, the resulting colonies are
countable . Because a sample may contain millions of cells per milliliter, it
must be diluted serially to achieve a plate with between 30 and 300
colonies . Samples with too many colonies are designated "Too Numerous To
Count" (TNTC), while those with fewer than 30 colonies are "Too Few To
Count" (TFTC) .
Section: Serial Dilution - Purpose
Source: HIMA 100 Lab 6 Assignment