BLOOD BANK ACTUAL CERTIFICATION
PAPER 2026 QUESTIONS WITH ANSWERS
FULL SOLUTION.
■ All blood that is drawn in CPD or CP2D can have its storage time
extended by adding an additive solution (also called Adsol and Adenine
Solution). When this is added within 72 hours of the unit's being drawn,
what will be the new expiration date. Answer- 42 days and no more.
■ If a unit of whole blood is drawn in CPDA-1, how long can it be
stored and used. Answer- 35 days
■ What is the minimum hemoglobin for allogeneic blood donations.
Answer- 12.5 g/dL
■ How much anticoagulant/preservative must be in a standard bag to
draw 450 mLs of whole blood. Answer- 63 mL
■ What is the minimum and maximum milliliters of blood that can be
drawn in a standard bag. Answer- 405 to 495 mLs
■ What is the minimum weight to be an allogeneic blood donor. Answer-
110 pounds
,■ What is the minimum hemoglobin and hematocrit for autologous
donations. Answer- 11 gm/dL and 33%
■ What is the pulse range for an allogeneic blood donor. Answer- 50 to
100 beats per minute
■ What is the systolic blood pressure limits for an allogeneic donor.
Answer- Less than or equal to 180
■ What is the upper age limit at which a person can have had hepatitis
and still be an allogeneic blood donor. Answer- 10 years of age
■ If a donor is hooked to a machine and only one component is
harvested from him, he is said to be donating by what technique.
Answer- Apheresis
■ A person who donates blood with the intent that he will receive his
own blood during a future surgery is called what kind of donor. Answer-
Autologous
■ If the antibody screen is positive after the addition of AHG and all
crossmatched units are compatible after AHG, what is a probable cause.
Answer- An alloantibody is present
, ■ When a clinically significant alloantibody is identified in the patient's
serum, what is done prior to transfusing the unit. Answer- Confirm that
the donor cells are negative for the antigen specific for the patient's
antibody by testing the donor cells with commercial antisera.
Perform a crossmatch using AHG
■ If a patient's sample is positive with all cells (screens, auto, and
crossmatches) after 37oC incubation, but negative after the addition of
AHG, what could be the cause. Answer- Rouleaux
■ If a patient's sample is negative with the screen cells and the
autocontrol, but all the crossmatched units are incompatible at all
phases, what may be the cause. Answer- The patient was incorrectly
ABO typed.
■ If a patient's sample is negative with the screen cells and the
autocontrol, but one of the crossmatched units is incompatible after
adding AHG, what may be the cause. Answer- The patient may have an
alloantibody to a low frequency antigen present on that one donor's cells
■ If a patient's sample is negative with the screen cells and the
autocontrol, but one of the crossmatched units is incompatible at
immediate spin, what may be the cause. Answer- The patient's serum
may have anti-M
PAPER 2026 QUESTIONS WITH ANSWERS
FULL SOLUTION.
■ All blood that is drawn in CPD or CP2D can have its storage time
extended by adding an additive solution (also called Adsol and Adenine
Solution). When this is added within 72 hours of the unit's being drawn,
what will be the new expiration date. Answer- 42 days and no more.
■ If a unit of whole blood is drawn in CPDA-1, how long can it be
stored and used. Answer- 35 days
■ What is the minimum hemoglobin for allogeneic blood donations.
Answer- 12.5 g/dL
■ How much anticoagulant/preservative must be in a standard bag to
draw 450 mLs of whole blood. Answer- 63 mL
■ What is the minimum and maximum milliliters of blood that can be
drawn in a standard bag. Answer- 405 to 495 mLs
■ What is the minimum weight to be an allogeneic blood donor. Answer-
110 pounds
,■ What is the minimum hemoglobin and hematocrit for autologous
donations. Answer- 11 gm/dL and 33%
■ What is the pulse range for an allogeneic blood donor. Answer- 50 to
100 beats per minute
■ What is the systolic blood pressure limits for an allogeneic donor.
Answer- Less than or equal to 180
■ What is the upper age limit at which a person can have had hepatitis
and still be an allogeneic blood donor. Answer- 10 years of age
■ If a donor is hooked to a machine and only one component is
harvested from him, he is said to be donating by what technique.
Answer- Apheresis
■ A person who donates blood with the intent that he will receive his
own blood during a future surgery is called what kind of donor. Answer-
Autologous
■ If the antibody screen is positive after the addition of AHG and all
crossmatched units are compatible after AHG, what is a probable cause.
Answer- An alloantibody is present
, ■ When a clinically significant alloantibody is identified in the patient's
serum, what is done prior to transfusing the unit. Answer- Confirm that
the donor cells are negative for the antigen specific for the patient's
antibody by testing the donor cells with commercial antisera.
Perform a crossmatch using AHG
■ If a patient's sample is positive with all cells (screens, auto, and
crossmatches) after 37oC incubation, but negative after the addition of
AHG, what could be the cause. Answer- Rouleaux
■ If a patient's sample is negative with the screen cells and the
autocontrol, but all the crossmatched units are incompatible at all
phases, what may be the cause. Answer- The patient was incorrectly
ABO typed.
■ If a patient's sample is negative with the screen cells and the
autocontrol, but one of the crossmatched units is incompatible after
adding AHG, what may be the cause. Answer- The patient may have an
alloantibody to a low frequency antigen present on that one donor's cells
■ If a patient's sample is negative with the screen cells and the
autocontrol, but one of the crossmatched units is incompatible at
immediate spin, what may be the cause. Answer- The patient's serum
may have anti-M
