MIMG 100L Exam Questions &
Answers (Grade A+)
Mixed culture -
correct answer ✅microbial culture consisting of two or more
species
Pure culture -
correct answer ✅microbial culture consisting of only a single
species
Streak plate method -
correct answer ✅cell density decreases until individual cells are
deposited, making colony forming units
Zigzag method -
correct answer ✅used for samples that do not have high cell
density
If colony is mixture of organisms, how do you purify? -
correct answer ✅Selective medias
Habitat controls -
correct answer ✅pH, temperature, pressure, nutritional elements
,MIMG 100L Exam Questions &
Answers (Grade A+)
Complex media examples -
correct answer ✅YTA - rich but lacks sugar
GYC - rich with glucose and CaCO3 (neutralize acid creating
clearings)
SYTA - sucrose, good for s. salivarius
Sabourauds agar -
correct answer ✅select for molds and yeasts, preference for high
acidic pH and high sugar content. Low pH inhibits bacterial growth
Cycloheximide (antibiotic) -
correct answer ✅inhibits growth of eukaryotes by interfering with
80S ribosome directed protein synthesis
Blood plates -
correct answer ✅alpha - growth but no clearing, oxidize
hemoglobin in green methemoglobin
beta - growth and clearing (lytic zone)
gamma - bacteria without hemolysin
, MIMG 100L Exam Questions &
Answers (Grade A+)
S. salivarius in different mediums -
correct answer ✅in SYTA: large, gummy, translucent, produces
capsules
in GYC: no sucrose to use, able to produce lactic acid that solubilizes
calcium carbonate in medium. creates clearing
Agar -
correct answer ✅complex polysaccharide, solidifying agent
Microorganisms -
correct answer ✅generally unicellular, cannot be seen clearly by
the naked eye, lack differentiated tissues
Origin of Life -
correct answer ✅3.8-3.9 BYA
All cells are composed of these attributes: -
correct answer ✅metabolism, growth, differentiation,
communication, movement, evolution
RNA World Hypothesis -
correct answer ✅single molecule of RNA was able to replicate
Answers (Grade A+)
Mixed culture -
correct answer ✅microbial culture consisting of two or more
species
Pure culture -
correct answer ✅microbial culture consisting of only a single
species
Streak plate method -
correct answer ✅cell density decreases until individual cells are
deposited, making colony forming units
Zigzag method -
correct answer ✅used for samples that do not have high cell
density
If colony is mixture of organisms, how do you purify? -
correct answer ✅Selective medias
Habitat controls -
correct answer ✅pH, temperature, pressure, nutritional elements
,MIMG 100L Exam Questions &
Answers (Grade A+)
Complex media examples -
correct answer ✅YTA - rich but lacks sugar
GYC - rich with glucose and CaCO3 (neutralize acid creating
clearings)
SYTA - sucrose, good for s. salivarius
Sabourauds agar -
correct answer ✅select for molds and yeasts, preference for high
acidic pH and high sugar content. Low pH inhibits bacterial growth
Cycloheximide (antibiotic) -
correct answer ✅inhibits growth of eukaryotes by interfering with
80S ribosome directed protein synthesis
Blood plates -
correct answer ✅alpha - growth but no clearing, oxidize
hemoglobin in green methemoglobin
beta - growth and clearing (lytic zone)
gamma - bacteria without hemolysin
, MIMG 100L Exam Questions &
Answers (Grade A+)
S. salivarius in different mediums -
correct answer ✅in SYTA: large, gummy, translucent, produces
capsules
in GYC: no sucrose to use, able to produce lactic acid that solubilizes
calcium carbonate in medium. creates clearing
Agar -
correct answer ✅complex polysaccharide, solidifying agent
Microorganisms -
correct answer ✅generally unicellular, cannot be seen clearly by
the naked eye, lack differentiated tissues
Origin of Life -
correct answer ✅3.8-3.9 BYA
All cells are composed of these attributes: -
correct answer ✅metabolism, growth, differentiation,
communication, movement, evolution
RNA World Hypothesis -
correct answer ✅single molecule of RNA was able to replicate
