ACS BIOCHEMISTRY FULL QUESTIONS AND CORRECT ANSWERS VERIFIED STUDY PACK

ACS BIOCHEMISTRY FULL QUESTIONS AND
CORRECT ANSWERS VERIFIED STUDY
PACK

●● Ramachandran plots
Answer: Φ = N-R
ψ = C-R
optimal angle to prevent steric hinderance


●● Protein structure of a helix, B sheet, reverse turn
Answer: a: LEMARKHQ
B: VICYFTW
RT: PSDNG


●● how many amino acids in alpha turn helix
Answer: 3.6


●● Super secondary structure
Answer: helix turn helix


●● parallel vs. antiparallel

,Answer: parallel wedges face same way
antiparallel face opposite


●● Isoelectric point (pI)
Answer: pH at which a particular molecule carries no net electrical
charge.


●● Protein Separation Techniques (ALL)
Answer: Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG


●● Protein Separation Techniques (H)
Answer: Homogenization
disrupt cells, sonicator, centrifuge


●● Protein Separation Techniques (S)
Answer: Salting out

, different proteins precipitate out at different salt concentrations


●● Protein Separation Techniques (A)
Answer: Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group


●● Protein Separation Techniques (I)
Answer: ion exchange
separation based on charge; + proteins stick to - beads


●● Protein Separation Techniques (SG)
Answer: Size Exclusion/Gel Filtration
separation based on size


●● Cuts (protein separation techniques)
Answer: cut out what dont need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep pellet)


●● Dialysis
Answer: protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration