MCB 252 FINAL EXAM AND STUDY GUIDE NEWEST 2026
TEST BANK| COMPLETE 450+ REAL EXAM QUESTIONS
AND CORRECT VERIFIED ANSWERS/ ALREADY GRADED
A+| MCB 252 FINAL EXAM
Which type of tissue described in lecture forms sheets of cells some of
which are involved in absorption, some in exchange of materials, some
in secretion and some in protection? ......ANSWER......epithelial tissue
As described in lecture, in general, in a classic adhesion experiment
different types of cells sort in such a way that cells from internal tissues
end up in the middle and cells from more external tissues end up
surrounding the cells form the more internal tissues.
......ANSWER......True
Which term most accurately and specifically describes the type of
library that produces a protein in E. coli? ......ANSWER......an expression
library
As described in lecture, apoptosis probably evolved in single celled
organisms before the emergence of multi-cellular organisms.
......ANSWER......False
pg. 1
,What type of experiment showed that C cadherin was required for cell
adhesion in developing Xenopus embryos? ......ANSWER......RNAi
inhibition of cadherin function
What type of experiment revealed which domain of cadherins mediated
the specificity of cadherin-cadherin adhesion? ......ANSWER......domain
swapping experiment
Different regions of the developing brain, which will each develop into
different final structures in the brain, express different cadherins.
......ANSWER......true
The catenins attach cadherins to the: ......ANSWER......actin cytoskeleton
You are studying a particular protein we'll call YFP (for Your Favorite
Protein). You hypothesize that YFP binds tightly to other proteins in the
cell and wish to identify those proteins. Which technique discussed in
lecture will most directly allow you to achieve your goal?
......ANSWER......a co-immunoprecipitation experiment
Which family of cell-cell adhesion molecules has approximately 750
family members in humans and is thought to play a role in wiring of the
human brain? ......ANSWER......ig-CAMs
Which family of cell-cell adhesion molecules is involved in leukocyte
"rolling" in blood vessels? ......ANSWER......Selectins
pg. 2
,As discussed in lecture, when we knockout (delete) your favorite gene
in mice, we start by engineering mouse embryonic stem cells. TRUE or
FALSE, we knock out (delete) both copies of your favorite gene in the
embryonic stem cells. ......ANSWER......False
Which of the following statements is TRUE with respect to our
discussion of the recombinant DNA construct used to generate a
"knockout" mouse? ......ANSWER......- If the construct integrates into a
chromosome by homologous recombination, 1 marker gene will be
integrated into the chromosome.
- If the construct integrates into a chromosome by random insertion
(non-homologous recombination), 2 marker genes will be integrated
into the chromosome.
Which type of junction prevents transmembrane proteins in the apical
part of the plasma membrane from diffusing into the basolateral part of
the plasma membrane? ......ANSWER......Tight Junctions
Adherens junction ......ANSWER......actin cytoskeleton of one cell to the
actin cytoskeleton of the neighboring cell
Desmosome ......ANSWER......intermediate filament cytoskeleton of one
cell to the intermediate filament cytoskeleton of a neighboring cell
pg. 3
, Hemi-desmosome ......ANSWER......intermediate filament cytoskeleton
of one cell to the extracellular matrix
Focal adhesion ......ANSWER......actin cytoskeleton on one cell to the
extracellular matrix
You inject one cell with a small fluorescent molecule (M.W. = 250
daltons) and find that after a short time that dye is found in the
neighboring cells, as diagrammed in the illustration below.
......ANSWER......These cells are connected to each other by gap
junctions.
In lecture we discussed the fact "n" represents the haploid DNA content
in an organism (the haploid amount of DNA found in each cell of that
organism) and that 2n is the diploid DNA content. What is the DNA
content in a human cell that is in the mitotic stage of the cell cycle?
......ANSWER......4n
In lecture we discussed the figure below. Which molecule results in
inhibition of the function carried out by the junction shown in this
figure if that molecule enters the cell? ......ANSWER......Ca++
You are studying a cell line in in which one cell cycle takes
approximately 24 hours. You perform FACS analysis on a population of
your dividing cells. Your cells are growing asynchronously meaning
different cells are in different stages of the cell cycle. Your FACS analysis
pg. 4
TEST BANK| COMPLETE 450+ REAL EXAM QUESTIONS
AND CORRECT VERIFIED ANSWERS/ ALREADY GRADED
A+| MCB 252 FINAL EXAM
Which type of tissue described in lecture forms sheets of cells some of
which are involved in absorption, some in exchange of materials, some
in secretion and some in protection? ......ANSWER......epithelial tissue
As described in lecture, in general, in a classic adhesion experiment
different types of cells sort in such a way that cells from internal tissues
end up in the middle and cells from more external tissues end up
surrounding the cells form the more internal tissues.
......ANSWER......True
Which term most accurately and specifically describes the type of
library that produces a protein in E. coli? ......ANSWER......an expression
library
As described in lecture, apoptosis probably evolved in single celled
organisms before the emergence of multi-cellular organisms.
......ANSWER......False
pg. 1
,What type of experiment showed that C cadherin was required for cell
adhesion in developing Xenopus embryos? ......ANSWER......RNAi
inhibition of cadherin function
What type of experiment revealed which domain of cadherins mediated
the specificity of cadherin-cadherin adhesion? ......ANSWER......domain
swapping experiment
Different regions of the developing brain, which will each develop into
different final structures in the brain, express different cadherins.
......ANSWER......true
The catenins attach cadherins to the: ......ANSWER......actin cytoskeleton
You are studying a particular protein we'll call YFP (for Your Favorite
Protein). You hypothesize that YFP binds tightly to other proteins in the
cell and wish to identify those proteins. Which technique discussed in
lecture will most directly allow you to achieve your goal?
......ANSWER......a co-immunoprecipitation experiment
Which family of cell-cell adhesion molecules has approximately 750
family members in humans and is thought to play a role in wiring of the
human brain? ......ANSWER......ig-CAMs
Which family of cell-cell adhesion molecules is involved in leukocyte
"rolling" in blood vessels? ......ANSWER......Selectins
pg. 2
,As discussed in lecture, when we knockout (delete) your favorite gene
in mice, we start by engineering mouse embryonic stem cells. TRUE or
FALSE, we knock out (delete) both copies of your favorite gene in the
embryonic stem cells. ......ANSWER......False
Which of the following statements is TRUE with respect to our
discussion of the recombinant DNA construct used to generate a
"knockout" mouse? ......ANSWER......- If the construct integrates into a
chromosome by homologous recombination, 1 marker gene will be
integrated into the chromosome.
- If the construct integrates into a chromosome by random insertion
(non-homologous recombination), 2 marker genes will be integrated
into the chromosome.
Which type of junction prevents transmembrane proteins in the apical
part of the plasma membrane from diffusing into the basolateral part of
the plasma membrane? ......ANSWER......Tight Junctions
Adherens junction ......ANSWER......actin cytoskeleton of one cell to the
actin cytoskeleton of the neighboring cell
Desmosome ......ANSWER......intermediate filament cytoskeleton of one
cell to the intermediate filament cytoskeleton of a neighboring cell
pg. 3
, Hemi-desmosome ......ANSWER......intermediate filament cytoskeleton
of one cell to the extracellular matrix
Focal adhesion ......ANSWER......actin cytoskeleton on one cell to the
extracellular matrix
You inject one cell with a small fluorescent molecule (M.W. = 250
daltons) and find that after a short time that dye is found in the
neighboring cells, as diagrammed in the illustration below.
......ANSWER......These cells are connected to each other by gap
junctions.
In lecture we discussed the fact "n" represents the haploid DNA content
in an organism (the haploid amount of DNA found in each cell of that
organism) and that 2n is the diploid DNA content. What is the DNA
content in a human cell that is in the mitotic stage of the cell cycle?
......ANSWER......4n
In lecture we discussed the figure below. Which molecule results in
inhibition of the function carried out by the junction shown in this
figure if that molecule enters the cell? ......ANSWER......Ca++
You are studying a cell line in in which one cell cycle takes
approximately 24 hours. You perform FACS analysis on a population of
your dividing cells. Your cells are growing asynchronously meaning
different cells are in different stages of the cell cycle. Your FACS analysis
pg. 4
