MNG3701 STUDY NOTES QUESTIONS
AND CORRECT ANSWERS 2026
LATEST PREP GUIDE VERIFIED A+
◉ What are the building blocks of a nucleotide?.
Answer: a sugar group, a phosphate group, and a nitrogenous base
◉ Chargaff's Rule.
Answer: A=T and C=G
- An organism has a GC content of 64% in its DNA. The percentage of
A would be: 18 and C would be 32
◉ Rank the given DNA sequences by their difficulty to separate into
single-stranded molecules by treatment with heat. Note: Most difficult
ranks 1st, least difficult ranks 3rd..
Answer: options:
1: CCGCATGGTCGCGTA
2: TTCAGCTATGTCTGA
3: TATGCTAAATGCTCT
◉ The following diagram represent DNA that is part of the RNA-coding
sequence of a transcription unit. The bottom strand is the template
strand. Give the sequence found on the RNA molecule transcribed form
this DNA and identify the 5' and 3' ends of the RNA
,5'ATAGGCGATGCCA'3
3'TATCCGCTACGGT'5 (temp).
Answer: 5' AUAGGCGAUGCCA 3'
◉ Write the consenus sequence for the following nucleotide sequences
AGGAGTT
AGCTATT
TGCAATA
ACGAAAA
TCCTAAT
TGCAATT
TGCAATT.
Answer: TGCAATT
◉ What is the polarity for the following coding strand. Template?.
Answer: 5' to 3', 3' to 5'
◉ Chromosomal DNA replication.
Answer: 1. DnaA proteins bind to the origin of replication, resulting in
the separation of the AT-rich region
2. DNA helicase breaks the hydrogen bonds between the DNA strands,
topoisomerases alleviate positive supercoiling, and single-strand binding
proteins hold the parental strands apart.
, 3. Primase synthesizes one RNA primer in the leading strand and many
RNA primers in the lagging strand, and DNA polymerase III synthesizes
the daughter strands of DNA.
PS 2 Q3
4. DNA polymerase I removes the RNA primers and fills in that region
with DNA, and DNA ligase covalently links Okazaki fragments
together.
5. Replication continues until the two replication forks meet on the other
side of the circular bacterial chromosome.
◉ dideoxy sequencing.
Answer: In dideoxy sequencing or site-directed mutagenesis, DNA
polymerase can be added after the annealing step, and the sample can be
incubated at lower temperatures that do not inactivate most forms of
DNA polymerase.
◉ During conjugation, transfer of genetic material is.
Answer: unidirectional
◉ Describe the structure of DNA.
Answer: -4 nitrogenous bases: A, C, G, and T
-Bases joined together by hydrogen bonds, G-C has 3 H-Bonds and A-T
has 2 H-Bonds
AND CORRECT ANSWERS 2026
LATEST PREP GUIDE VERIFIED A+
◉ What are the building blocks of a nucleotide?.
Answer: a sugar group, a phosphate group, and a nitrogenous base
◉ Chargaff's Rule.
Answer: A=T and C=G
- An organism has a GC content of 64% in its DNA. The percentage of
A would be: 18 and C would be 32
◉ Rank the given DNA sequences by their difficulty to separate into
single-stranded molecules by treatment with heat. Note: Most difficult
ranks 1st, least difficult ranks 3rd..
Answer: options:
1: CCGCATGGTCGCGTA
2: TTCAGCTATGTCTGA
3: TATGCTAAATGCTCT
◉ The following diagram represent DNA that is part of the RNA-coding
sequence of a transcription unit. The bottom strand is the template
strand. Give the sequence found on the RNA molecule transcribed form
this DNA and identify the 5' and 3' ends of the RNA
,5'ATAGGCGATGCCA'3
3'TATCCGCTACGGT'5 (temp).
Answer: 5' AUAGGCGAUGCCA 3'
◉ Write the consenus sequence for the following nucleotide sequences
AGGAGTT
AGCTATT
TGCAATA
ACGAAAA
TCCTAAT
TGCAATT
TGCAATT.
Answer: TGCAATT
◉ What is the polarity for the following coding strand. Template?.
Answer: 5' to 3', 3' to 5'
◉ Chromosomal DNA replication.
Answer: 1. DnaA proteins bind to the origin of replication, resulting in
the separation of the AT-rich region
2. DNA helicase breaks the hydrogen bonds between the DNA strands,
topoisomerases alleviate positive supercoiling, and single-strand binding
proteins hold the parental strands apart.
, 3. Primase synthesizes one RNA primer in the leading strand and many
RNA primers in the lagging strand, and DNA polymerase III synthesizes
the daughter strands of DNA.
PS 2 Q3
4. DNA polymerase I removes the RNA primers and fills in that region
with DNA, and DNA ligase covalently links Okazaki fragments
together.
5. Replication continues until the two replication forks meet on the other
side of the circular bacterial chromosome.
◉ dideoxy sequencing.
Answer: In dideoxy sequencing or site-directed mutagenesis, DNA
polymerase can be added after the annealing step, and the sample can be
incubated at lower temperatures that do not inactivate most forms of
DNA polymerase.
◉ During conjugation, transfer of genetic material is.
Answer: unidirectional
◉ Describe the structure of DNA.
Answer: -4 nitrogenous bases: A, C, G, and T
-Bases joined together by hydrogen bonds, G-C has 3 H-Bonds and A-T
has 2 H-Bonds
