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samenvatting LABO biotechnologie

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Samenvatting van het labo deel van het vak biotechnologie. In dit document staan alle gebruikte formules, belangrijke theorievragen en alle info over de gebruikte buffers in het lab. Zeer examengevoelige onderwerpen dus. OPGEPAST: deze omvat niet alle theorie, aangezien veel theorie terugkomt in de samenvatting van het theoriedeel. Daarom raad ik aan om deze enkel te kopen indien je je theorie goed kent of de voordeelbundel samen met de theorie te kopen.

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Institution
UC Leuven-Limburg
Course
Biotechnologie (MBT43C)

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BIOTECHNOLOGIE




BIOTECHNOLOGIE
LAB
2FBT – AJ 2025-26

, BIOTECHNOLOGIE


Formules

Verdunningen

C1*V1=C2*V2
- C₁ = stockconcentratie
- V₁ = stockvolume
- C₂ = gewenste concentratie
- V₂ = eindvolume




DNA-concentratie

Nanofotometer:

A260 = DNA

1 OD260=50 µg/mL dsDNA → DNA = A260* 50 (*DF)

1 OD260=38 µg/mL ssDNA → DNA = A260* 38

1 OD260=40 µg/mL ssRNA → RNA = A260* 40

Totale opbrengst (µg) = [DNA] * Vtotaal

Massa1DNA template= (# bp* 650g/mol)/ 6,022*1023



Zuiverheid DNA

A280= eiwitten

A230= organische verbindingen en zouten

A260 / A280

Voor DNA: 1.8-2.0

Voor RNA: >2.0

A260 / A230

Voor DNA: 2.0-2.2

Voor RNA: 2.0-2.4



PCR

Tm = 4*(G+C)+2*(A+T)

Tm (>18nt)= 64,9+ 41*[(#GC-16,4)/ totaal # nt]

Ta= Tm- 3 tot 5°C

Mr primer= [(#A=313,22)+(#C* 298,18)+(#G*329,22)+ (#T*304,21)] + 18
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Institution
UC Leuven-Limburg
Study
biomedische laboratoriumtechnologie
Course
Biotechnologie (MBT43C)
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Uploaded on
June 7, 2026
Number of pages
6
Written in
2025/2026
Type
SUMMARY

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  • pcr
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overzichtelijke samenvattingen FBT

Al die samenvattingen met 1 doorlopende tekst, zonder titels etc.. beu? Maak kennis met mijn samenvattingen met structuur en overzichtelijke kaders voor vergelijkingen tussen 2/ meerdere dingen, duidelijek stappenplannen, visualisaties...! Moesten er vragen zijn mogen jullie deze gerust stellen! :) Alvast heel veel succes x

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