BIOL-134 Lab Exam | Questions and Answers |
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Terms in this set (40)
Why was the riboflavin solution Riboflavin absorbancy was the most around 450
yellow and the NaCl solution clear? nanometer wavelength which is absorbing blue
and the lowest was around 600 nanometers which
is reflecting yellow. NaCl solution is clear because
it does not absorb visible light.
What do we know about It is a gram negative bacteria that can survive in
Pseudomonas aeruginosa? diverse environments, can cause infections,
resistant to many antibiotics
Why are proteinase K and RNase Proteinase K is used to break down the cell
used in the isolation of DNA? membrane, RNase degrades RNA. They remove
contaminants so you get a cleaner sample of
genomic DNA.
How is genomic DNA removed from Genomic DNA is removed during plasmid isolation
solution during the isolation of by raising the pH to denature all DNA; when the
plasmid DNA? solution is neutralized, the large, nicked genomic
DNA becomes insoluble and is pelleted by
centrifugation, while the small, supercoiled plasmid
DNA stays in solution in the supernatant
, Pellet dense material that collects at the bottom of
thetube
Supernatant liquid that remains in solution
What are the properties of restriction Restriction enzymes cleave double stranded DNA
enzymes? at specific target sequences. After the DNA has
been cut the fragments can be joined to other
DNA fragments to create recombinant DNA
molecules. The process of joining the molecules is
called ligation
Do restriction enzymes target Restriction enzymes typically cleave DNA at
specific sequences? Why are they palindromic sequences; the sequence is the same
useful in gene cloning? from 5' to 3' on both strands. These enzymes are
isolated from bacteria, where they may function as
a protection against viruses.
What are sticky ends? overhanging sequences at the site of the restriction
enzyme cut
What are the properties of plasmids Plasmids used for cloning have been genetically
used for gene cloning? engineered to facilitate the cloning process. They
contain genes for antibiotic resistance and
restriction enzyme sites to facilitate cloning. They
may also contain genes that allow for identification
of recombinant plasmids.
What is a multiple cloning site? region that contains several restriction enzyme
sites located adjacent to one another; this helps to
facilitate the cloning.
Verified Solutions | 2026 Edition | Pass
Guaranteed
Save
Terms in this set (40)
Why was the riboflavin solution Riboflavin absorbancy was the most around 450
yellow and the NaCl solution clear? nanometer wavelength which is absorbing blue
and the lowest was around 600 nanometers which
is reflecting yellow. NaCl solution is clear because
it does not absorb visible light.
What do we know about It is a gram negative bacteria that can survive in
Pseudomonas aeruginosa? diverse environments, can cause infections,
resistant to many antibiotics
Why are proteinase K and RNase Proteinase K is used to break down the cell
used in the isolation of DNA? membrane, RNase degrades RNA. They remove
contaminants so you get a cleaner sample of
genomic DNA.
How is genomic DNA removed from Genomic DNA is removed during plasmid isolation
solution during the isolation of by raising the pH to denature all DNA; when the
plasmid DNA? solution is neutralized, the large, nicked genomic
DNA becomes insoluble and is pelleted by
centrifugation, while the small, supercoiled plasmid
DNA stays in solution in the supernatant
, Pellet dense material that collects at the bottom of
thetube
Supernatant liquid that remains in solution
What are the properties of restriction Restriction enzymes cleave double stranded DNA
enzymes? at specific target sequences. After the DNA has
been cut the fragments can be joined to other
DNA fragments to create recombinant DNA
molecules. The process of joining the molecules is
called ligation
Do restriction enzymes target Restriction enzymes typically cleave DNA at
specific sequences? Why are they palindromic sequences; the sequence is the same
useful in gene cloning? from 5' to 3' on both strands. These enzymes are
isolated from bacteria, where they may function as
a protection against viruses.
What are sticky ends? overhanging sequences at the site of the restriction
enzyme cut
What are the properties of plasmids Plasmids used for cloning have been genetically
used for gene cloning? engineered to facilitate the cloning process. They
contain genes for antibiotic resistance and
restriction enzyme sites to facilitate cloning. They
may also contain genes that allow for identification
of recombinant plasmids.
What is a multiple cloning site? region that contains several restriction enzyme
sites located adjacent to one another; this helps to
facilitate the cloning.
