MLS 412: CLS Theory, Application, & Correlation –
Exam 2 Pre- and Post- Session Exercises 2026:
Complete Exam Prep with Questions, Answers &
Detailed Explanations
If a patient has severe anemia with compensatory Bart's
hemoglobin and absent Heinz bodies, what should you
suspect?
alpha thalassemia major/Hydrops fetalis
If a patient has moderate anemia with targets, Heinz bodies,
and stippled RBCs present, compensatory Hgb H, and a
variable serum ferritin, what should you suspect?
alpha thalassemia intermedia/Hemoglobin H disease
If a patient has mild anemia, no compensatory Hgb, present
targets and stippled RBCs, along with a normal serum ferritin,
what should you suspect?
,alpha thalassemia minor
If a patient has severe anemia, compensatory Hgb F, present
targets, stippled RBCs, and Heinz bodies, along with an
increased serum ferritin, what should you suspect?
beta thalassemia major/Cooley's anemia
If a patient has mild anemia with compensatory Hgb A2,
targets and stippled RBCs, and a normal serum ferritin, what
should you suspect?
beta thalassemia minor
If a patient has mild anemia, targets, C crystals, and abnormal
Hgb C, what should you suspect?
Hgb C disease
If a patient has no anemia, targets, and abnormal Hgb C, what
should you suspect?
Hgb C trait
If a patient has moderate-severe anemia, targets, nucRBCs,
Sickle cells, C crystals, bizzare RBCs, along with abnormal Hgb
S and Hgb C, what should you suspect?
Hgb SC disease
If a patient has severe anemia, targets, nucRBCs, Sickle cells,
and abnormal Hgb S, what should you suspect?
Sickle cell disease
,If a patient has no anemia, targets, and abnormal Hgb S, what
should you suspect?
Sickle cell trait
On alkaline electrophoresis on cellulose acetate at pH 8.6, the
correct order of migration of hemoglobin from the application
point (cathode to anode) (slowest to fastest) is:
C, S, F, A
Which test would you expect to be negative if a patient has a
positive DAT?
a. autocontrol
b. Coombs phase Ag testing
c. IS XM
d. weak D test
IS XM; a positive DAT invalidates any test that uses patient RBCs
at the AHG phase of testing
Why can reaction strength vary on antibody panels?
- antigen variability
- dosage
- multiple antibodies are present
The antibody screen on a patient is positive with two of three
screen cells at the immediate spin and AHG phases of testing.
An auto control tested with the antibody screen is negative. A
selected cell panel is performed and anti-P1 is identified. All
other antibodies are excluded. Repeating the antibody screen
, at AHG using the prewarm technique eliminates the positive
reactions. The most likely explanation for these results is a(n):
insignificant cold reacting alloanti-P1
The criteria that must be met in order to identify an antibody
with a confidence of 95% probability includes (two / three)
antigen positive cells that react and (two / three) antigen
negative cells that fail to react.
three; three
It appears a patient's plasma contains both anti-S and anti-
Jka. To provide 95% confidence in the proper identification of
the antibodies, what should the cell and serum results look
like?
must have a positive anti-S alone, a positive anti-Jka alone, and
a negative all alone (the three-three rule)
What percentage of the donor population would be
compatible with a patient who has anti-Fya? (The antigen
frequency of Fya is 65%)
35% (just subtract from 100%)
What type(s) of red blood cells is (are) acceptable to transfuse
to an AB Rh-negative patient?
A=, B=, AB=, and O=
Select, in order of choice (if applicable), which alternative
blood group(s) should be transfused when type specific
plasma is not available for a Group B patient:
Exam 2 Pre- and Post- Session Exercises 2026:
Complete Exam Prep with Questions, Answers &
Detailed Explanations
If a patient has severe anemia with compensatory Bart's
hemoglobin and absent Heinz bodies, what should you
suspect?
alpha thalassemia major/Hydrops fetalis
If a patient has moderate anemia with targets, Heinz bodies,
and stippled RBCs present, compensatory Hgb H, and a
variable serum ferritin, what should you suspect?
alpha thalassemia intermedia/Hemoglobin H disease
If a patient has mild anemia, no compensatory Hgb, present
targets and stippled RBCs, along with a normal serum ferritin,
what should you suspect?
,alpha thalassemia minor
If a patient has severe anemia, compensatory Hgb F, present
targets, stippled RBCs, and Heinz bodies, along with an
increased serum ferritin, what should you suspect?
beta thalassemia major/Cooley's anemia
If a patient has mild anemia with compensatory Hgb A2,
targets and stippled RBCs, and a normal serum ferritin, what
should you suspect?
beta thalassemia minor
If a patient has mild anemia, targets, C crystals, and abnormal
Hgb C, what should you suspect?
Hgb C disease
If a patient has no anemia, targets, and abnormal Hgb C, what
should you suspect?
Hgb C trait
If a patient has moderate-severe anemia, targets, nucRBCs,
Sickle cells, C crystals, bizzare RBCs, along with abnormal Hgb
S and Hgb C, what should you suspect?
Hgb SC disease
If a patient has severe anemia, targets, nucRBCs, Sickle cells,
and abnormal Hgb S, what should you suspect?
Sickle cell disease
,If a patient has no anemia, targets, and abnormal Hgb S, what
should you suspect?
Sickle cell trait
On alkaline electrophoresis on cellulose acetate at pH 8.6, the
correct order of migration of hemoglobin from the application
point (cathode to anode) (slowest to fastest) is:
C, S, F, A
Which test would you expect to be negative if a patient has a
positive DAT?
a. autocontrol
b. Coombs phase Ag testing
c. IS XM
d. weak D test
IS XM; a positive DAT invalidates any test that uses patient RBCs
at the AHG phase of testing
Why can reaction strength vary on antibody panels?
- antigen variability
- dosage
- multiple antibodies are present
The antibody screen on a patient is positive with two of three
screen cells at the immediate spin and AHG phases of testing.
An auto control tested with the antibody screen is negative. A
selected cell panel is performed and anti-P1 is identified. All
other antibodies are excluded. Repeating the antibody screen
, at AHG using the prewarm technique eliminates the positive
reactions. The most likely explanation for these results is a(n):
insignificant cold reacting alloanti-P1
The criteria that must be met in order to identify an antibody
with a confidence of 95% probability includes (two / three)
antigen positive cells that react and (two / three) antigen
negative cells that fail to react.
three; three
It appears a patient's plasma contains both anti-S and anti-
Jka. To provide 95% confidence in the proper identification of
the antibodies, what should the cell and serum results look
like?
must have a positive anti-S alone, a positive anti-Jka alone, and
a negative all alone (the three-three rule)
What percentage of the donor population would be
compatible with a patient who has anti-Fya? (The antigen
frequency of Fya is 65%)
35% (just subtract from 100%)
What type(s) of red blood cells is (are) acceptable to transfuse
to an AB Rh-negative patient?
A=, B=, AB=, and O=
Select, in order of choice (if applicable), which alternative
blood group(s) should be transfused when type specific
plasma is not available for a Group B patient:
