BOC MICROBIOLOGY TEST BANK FULL
QUESTIONS AND SOLUTIONS VERIFIED
PASS GUARANTEED SUCCESS
●● the most appropriate method for collecting a urine specimen from a
patient with an indwelling catheter?
Answer: aspirate urine aseptically from the catheter tubing
●● which of the following groups of specimens would be acceptable for
anaerobic culture?
Answer: pleural fluid, brain abcess fluid
●● sodium polyanetholsulfonate (SPS) is used as an anticoagulant for
blood cultures because it:
Answer: inactivates neutrophils and components of serum complement
●● the optimal collection of a wound specimen for culture of anaerobic
organisms is a:
Answer: syringe filled with pus, obtained before administration of
antibiotics
●● the most important variable in the recovery of organisms in adult
patients with bacteremia (bacterial sepsis) is:
,Answer: the recommended volume of blood ordered
●● virus transport medium should contain agents that:
Answer: inhibit bacterial and fungal growth
●● a bronchoscopy sample with the request culture for Legionella is sent
to the lab. the correct plating protocol is:
Answer: culture on buffered charcoal yeast extract agar with antibiotics
●● a community hospital microbiology lab is processing significant
numbers of stool cultures because of an outbreak of diarrhea following
heavy rains and flooding in the county. a media that should be
incorporated in the plating protocol is:
Answer: thiosulfate citrate bile salts sucrose for Vibrio spp.
●● a male urethral discharge specimen submitted for culture should be
inoculated to:
Answer: chocolate and modified Thayer-Martin agars
●● the steam autoclave method of sterilization requires
Answer: 15# pressure 15 minutes
●● Which selective medium is used for the isolation of gram-positive
microorganisms?
,Answer: Columbia CNA with 5% sheep blood
●● Campylobacter jejuni isolation requires the fecal specimen be:
Answer: inoculated onto selective plating media and incubated in
reduced oxygen with added CO2 at 42C
●● which of the following specimen requests is acceptable?
Answer: urine culture for acid-fast bacilli
●● a CSF containing only 2 drops of CSF was collected by a lumbar
puncture from a 25 year old male, and submitted for stat gram stain and
culture. The direct specimen gram stain was reported as many
neutrophils and no microorganisms seen. The remaining drop of CSF
should be inoculated to:
Answer: chocolate agar
●● a diabetic food swab from an 82 year old woman with recurrent
infections is submitted for culture. The gram stain reveals:
Many neutrophils no squamous cells
many gram-neg bacilli
Many gram-pos cocci in chains
Dr requests that all pathogens be worked up, in addition to sheep blood,
chocolate and MacConkey, the tech may also process a:
Answer: CNA agar plate
, ●● which of the following is the most appropriate specimen source and
primary media selection?
Answer: endocervical; chocolate, Martin Lewis
●● which of the following is the most appropriate organism and media
combo?
Answer: Yersinia enterocolitica-CIN agar
●● a gram stain from a hand wound swab:
moderate neutrophils, no squamous, moderat GP cocci in clusters,
moderate GN bacilli
the appropriate media that will selectively isolate each org?
Answer: CNA, MacConkey
●● an organism that must be incubated in a microaerophilic environment
for optimal recovery is:
Answer: C. jejuni
●● Vibrio parahaemolyticus is best isolated from feces on:
Answer: TCBS
QUESTIONS AND SOLUTIONS VERIFIED
PASS GUARANTEED SUCCESS
●● the most appropriate method for collecting a urine specimen from a
patient with an indwelling catheter?
Answer: aspirate urine aseptically from the catheter tubing
●● which of the following groups of specimens would be acceptable for
anaerobic culture?
Answer: pleural fluid, brain abcess fluid
●● sodium polyanetholsulfonate (SPS) is used as an anticoagulant for
blood cultures because it:
Answer: inactivates neutrophils and components of serum complement
●● the optimal collection of a wound specimen for culture of anaerobic
organisms is a:
Answer: syringe filled with pus, obtained before administration of
antibiotics
●● the most important variable in the recovery of organisms in adult
patients with bacteremia (bacterial sepsis) is:
,Answer: the recommended volume of blood ordered
●● virus transport medium should contain agents that:
Answer: inhibit bacterial and fungal growth
●● a bronchoscopy sample with the request culture for Legionella is sent
to the lab. the correct plating protocol is:
Answer: culture on buffered charcoal yeast extract agar with antibiotics
●● a community hospital microbiology lab is processing significant
numbers of stool cultures because of an outbreak of diarrhea following
heavy rains and flooding in the county. a media that should be
incorporated in the plating protocol is:
Answer: thiosulfate citrate bile salts sucrose for Vibrio spp.
●● a male urethral discharge specimen submitted for culture should be
inoculated to:
Answer: chocolate and modified Thayer-Martin agars
●● the steam autoclave method of sterilization requires
Answer: 15# pressure 15 minutes
●● Which selective medium is used for the isolation of gram-positive
microorganisms?
,Answer: Columbia CNA with 5% sheep blood
●● Campylobacter jejuni isolation requires the fecal specimen be:
Answer: inoculated onto selective plating media and incubated in
reduced oxygen with added CO2 at 42C
●● which of the following specimen requests is acceptable?
Answer: urine culture for acid-fast bacilli
●● a CSF containing only 2 drops of CSF was collected by a lumbar
puncture from a 25 year old male, and submitted for stat gram stain and
culture. The direct specimen gram stain was reported as many
neutrophils and no microorganisms seen. The remaining drop of CSF
should be inoculated to:
Answer: chocolate agar
●● a diabetic food swab from an 82 year old woman with recurrent
infections is submitted for culture. The gram stain reveals:
Many neutrophils no squamous cells
many gram-neg bacilli
Many gram-pos cocci in chains
Dr requests that all pathogens be worked up, in addition to sheep blood,
chocolate and MacConkey, the tech may also process a:
Answer: CNA agar plate
, ●● which of the following is the most appropriate specimen source and
primary media selection?
Answer: endocervical; chocolate, Martin Lewis
●● which of the following is the most appropriate organism and media
combo?
Answer: Yersinia enterocolitica-CIN agar
●● a gram stain from a hand wound swab:
moderate neutrophils, no squamous, moderat GP cocci in clusters,
moderate GN bacilli
the appropriate media that will selectively isolate each org?
Answer: CNA, MacConkey
●● an organism that must be incubated in a microaerophilic environment
for optimal recovery is:
Answer: C. jejuni
●● Vibrio parahaemolyticus is best isolated from feces on:
Answer: TCBS
