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BIOS 255 LAB PRACTICAL EXAM 2 (Latest Update 2025 / 2026) Anatomy and Physiology IV with Lab | Questions & Answers | 100% Correct | Grade A – Chamberlain

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BIOS 255 LAB PRACTICAL EXAM 2 (Latest Update 2025 / 2026) Anatomy and Physiology IV with Lab | Questions & Answers | 100% Correct | Grade A – Chamberlain

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BIOS 255 LAB PRACTICAL EXAM 2 (Latest
Update ) Anatomy and
Physiology IV with Lab | Questions &
Answers | 100% Correct | Grade A –
Chamberlain

1. Which part of the microscope holds the slide in place?
r. r. r. r. r. r. r. r. r.




A. Stage clip
r. r.




B. Diaphragm
r.




C. Revolving nosepiece
r. r.




D. Coarse adjustment knob
r. r. r.




Correct Answer: A r. r.




Rationale: Stage clips secure the slide to prevent movement during
r. r. r. r. r. r. r. r. r.




observation.
r.




2. What is the primary purpose of a dissecting microscope?
r. r. r. r. r. r. r. r. r.




A. Observe large specimens in 3D
r. r. r. r. r.




B. View organelles
r. r.




C. Count cells in liquid
r. r. r. r.




D. Measure ATP production
r. r. r.




Correct Answer: A r. r.




Rationale: Dissecting microscopes provide low magnification and
r. r. r. r. r. r.




depth for whole specimens.
r. r. r. r.

,3. In Gram staining, what is the role of alcohol?
r. r. r. r. r. r. r. r. r.




A. Decolorize Gram-negative bacteria
r. r. r.




B. Primary stain
r. r.




C. Counterstain
r.




D. Mordantr.




Correct Answer: A r. r.




Rationale: Alcohol removes crystal violet from Gram-negative cells
r. r. r. r. r. r. r.




while Gram-positive retain it.
r. r. r. r.




4. The correct sequence for preparing a wet mount is:
r. r. r. r. r. r. r. r. r.




A. Place drop of water → add specimen → coverslip at angle
r. r. r. r. r. r. r. r. r. r. r.




B. Coverslip first → water → specimen
r. r. r. r. r. r.




C. Dry specimen → coverslip → water
r. r. r. r. r. r.




D. Water only, no coverslip
r. r. r. r.




Correct Answer: A r. r.




Rationale: Proper placement prevents bubbles and allows
r. r. r. r. r. r.




observation of live cells.
r. r. r. r.




5. Which type of cell division produces two identical daughter
r. r. r. r. r. r. r. r. r.




cells?
r.




A. Mitosis
r.




B. Meiosis
r.




C. Binary fission in bacteria only
r. r. r. r. r.




D. Buddingr.




Correct Answer: A r. r.

,Rationale: Mitosis ensures genetically identical cells for growth
r. r. r. r. r. r. r.




and repair.
r. r.




6. Benedict’s reagent changes color when heated in presence of:
r. r. r. r. r. r. r. r. r.




A. Reducing sugars
r. r.




B. Starch
r.




C. Proteins
r.




D. Lipids r.




Correct Answer: A r. r.




Rationale: Reducing sugars reduce copper ions in Benedict’s
r. r. r. r. r. r. r.




solution, producing color.
r. r. r.




7. A negative stain is useful because it:
r. r. r. r. r. r. r.




A. Stains background, leaving cells unstained
r. r. r. r. r.




B. Kills cells
r. r.




C. Stains only nuclei
r. r. r.




D. Measures motility
r. r.




Correct Answer: A r. r.




Rationale: Negative stains provide contrast without affecting cell
r. r. r. r. r. r. r.




structure.
r.




8. Methylene blue is used in microbiology primarily to:
r. r. r. r. r. r. r. r.




A. Stain nuclei
r. r.




B. Detect lipids
r. r.




C. Visualize starch
r. r.

, D. Decolorize Gram-negative bacteria
r. r. r.




Correct Answer: A r. r.




Rationale: Methylene blue binds nucleic acids for nucleus
r. r. r. r. r. r. r.




visualization.
r.




9. The function of the coarse adjustment knob is to:
r. r. r. r. r. r. r. r. r.




A. Bring specimen roughly into focus
r. r. r. r. r.




B. Fine-tune focus at high power
r. r. r. r. r.




C. Adjust light intensity
r. r. r.




D. Change magnification
r. r.




Correct Answer: A r. r.




Rationale: Coarse adjustment is used for initial focusing at low
r. r. r. r. r. r. r. r. r.




magnification.
r.




10. The function of the fine adjustment knob is:
r. r. r. r. r. r. r. r.




A. Sharpen image at high magnification
r. r. r. r. r.




B. Move the slide horizontally
r. r. r. r.




C. Change light intensity
r. r. r.




D. Hold the slide
r. r. r.




Correct Answer: A r. r.




Rationale: Fine adjustment allows precise focusing after coarse
r. r. r. r. r. r. r.




adjustment.
r.




11. Which bacteria arrangement forms clusters like grapes?
r. r. r. r. r. r. r.

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