Pearson Edexcel Level 3 GCE
Thursday 5 June 2025
Afternoon (Time: 1 hour 45 minutes) Paper
reference 9BI0/01
Biology B
Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 Active transport is one type of cell transport mechanism.
(a) What happens during active transport?
(1)
A ATP is hydrolysed releasing energy
B ATP is hydrolysed using energy
C ATP is phosphorylated releasing energy
D ATP is phosphorylated using energy
(b) Which substance is transported against a concentration gradient by active
transport?
(1)
A hydrophilic molecule
B large hydrophobic molecule
C small hydrophobic molecule
D water
2
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, (c) The graph shows the change in concentration of molecule A and molecule B, as
they are transported into the cytoplasm of a cell.
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
molecule A
Concentration of molecules
in the cytoplasm molecule B
0 10 20 30
Time / mins
Explain the differences in the changes in concentration of molecule A and
molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 4 marks)
3
*P74459A0336* Turn over
, 2 The diagram shows part of cellular respiration.
glucose
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glycolysis
molecule X
link reaction
and
Krebs cycle
carbon dioxide
(a) Which is molecule X?
(1)
A glycerate 3-phosphate
B glycogen
C lactate
D pyruvate
(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1)
Glycolysis Link reaction Krebs cycle
A cytoplasm cytoplasm cytoplasm
B cytoplasm mitochondria mitochondria
C mitochondria cytoplasm cytoplasm
D mitochondria mitochondria mitochondria
4
*P74459A0436*
Thursday 5 June 2025
Afternoon (Time: 1 hour 45 minutes) Paper
reference 9BI0/01
Biology B
Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 Active transport is one type of cell transport mechanism.
(a) What happens during active transport?
(1)
A ATP is hydrolysed releasing energy
B ATP is hydrolysed using energy
C ATP is phosphorylated releasing energy
D ATP is phosphorylated using energy
(b) Which substance is transported against a concentration gradient by active
transport?
(1)
A hydrophilic molecule
B large hydrophobic molecule
C small hydrophobic molecule
D water
2
*P74459A0236*
, (c) The graph shows the change in concentration of molecule A and molecule B, as
they are transported into the cytoplasm of a cell.
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
molecule A
Concentration of molecules
in the cytoplasm molecule B
0 10 20 30
Time / mins
Explain the differences in the changes in concentration of molecule A and
molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 4 marks)
3
*P74459A0336* Turn over
, 2 The diagram shows part of cellular respiration.
glucose
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glycolysis
molecule X
link reaction
and
Krebs cycle
carbon dioxide
(a) Which is molecule X?
(1)
A glycerate 3-phosphate
B glycogen
C lactate
D pyruvate
(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1)
Glycolysis Link reaction Krebs cycle
A cytoplasm cytoplasm cytoplasm
B cytoplasm mitochondria mitochondria
C mitochondria cytoplasm cytoplasm
D mitochondria mitochondria mitochondria
4
*P74459A0436*
