Pearson Edexcel Level 3 GCE
Monday 12 May 2025
Morning (Time: 1 hour 30 minutes) Paper
reference 8BI0/01
Biology B
Advanced Subsidiary
PAPER 1: Core Cellular Biology and Microbiology
, Some questions must be answered with a cross in a box . If you change your mind about an
answer, put a line through the box and then mark your new answer with a cross .
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
1 Some carbohydrates are composed of three monosaccharides. These are
called trisaccharides.
Raffinose is a trisaccharide composed of glucose, galactose and fructose.
(a) Complete the diagram to show the structure of an alpha glucose molecule.
(2)
CH2OH
C O
H
C C
C C
(b) Name the bond that joins monosaccharides together.
(1)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
(c) The three monosaccharides present in raffinose each have a molecular mass
of 180.
The relative atomic mass of the element hydrogen is 1 and the relative atomic
mass of the element oxygen is 16.
Calculate the molecular mass of raffinose.
(1)
Answer ......................... ............... . . . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 4 marks)
2
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, 2 During the S phase of the cell cycle, DNA is synthesised by
semi‑conservative replication.
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
The diagram shows part of a DNA molecule.
bases J and K
bases L and M
bond E
bond D bond F
(a) (i) Name the base pairs labelled J and K, L and M.
(2)
J and K . .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
L and M . . .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................... ................ . . . . . . . . . . . . . . . . . . . .
(ii) Name the bonds labelled D, E and F.
(2)
Bond D. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
Bond E. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
Bond F... .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
3
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, (iii) Part of a DNA molecule consists of 840 bases. Of these, 136 are base J.
Calculate the percentage of bases that are M in this DNA molecule.
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Give your answer to two decimal places.
(1)
Answer ............................................... . . . . . . . . . . . . . . . %
(b) State what is meant by the term semi‑conservative replication.
(1)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 2 = 6 marks)
4
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Monday 12 May 2025
Morning (Time: 1 hour 30 minutes) Paper
reference 8BI0/01
Biology B
Advanced Subsidiary
PAPER 1: Core Cellular Biology and Microbiology
, Some questions must be answered with a cross in a box . If you change your mind about an
answer, put a line through the box and then mark your new answer with a cross .
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
1 Some carbohydrates are composed of three monosaccharides. These are
called trisaccharides.
Raffinose is a trisaccharide composed of glucose, galactose and fructose.
(a) Complete the diagram to show the structure of an alpha glucose molecule.
(2)
CH2OH
C O
H
C C
C C
(b) Name the bond that joins monosaccharides together.
(1)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
(c) The three monosaccharides present in raffinose each have a molecular mass
of 180.
The relative atomic mass of the element hydrogen is 1 and the relative atomic
mass of the element oxygen is 16.
Calculate the molecular mass of raffinose.
(1)
Answer ......................... ............... . . . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 4 marks)
2
*P78689A0232*
, 2 During the S phase of the cell cycle, DNA is synthesised by
semi‑conservative replication.
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
The diagram shows part of a DNA molecule.
bases J and K
bases L and M
bond E
bond D bond F
(a) (i) Name the base pairs labelled J and K, L and M.
(2)
J and K . .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
L and M . . .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................... ................ . . . . . . . . . . . . . . . . . . . .
(ii) Name the bonds labelled D, E and F.
(2)
Bond D. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
Bond E. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
Bond F... .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ........................................................................................................................................................... . . . . . . . . . . . . . . . . . . . .
3
*P78689A0332* Turn over
, (iii) Part of a DNA molecule consists of 840 bases. Of these, 136 are base J.
Calculate the percentage of bases that are M in this DNA molecule.
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
Give your answer to two decimal places.
(1)
Answer ............................................... . . . . . . . . . . . . . . . %
(b) State what is meant by the term semi‑conservative replication.
(1)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 2 = 6 marks)
4
*P78689A0432*
