BIO 171 Microbiology: BIO 171 lab 1-9 Notebook
Lab Notebook 1-9: Complete Updated Solution
Lab 1 Notebook
Title: Introduction to Medical Microbiology
Objective: To establish an organized template for keeping experimental records, procedures,
and results
Cultivation of lab samples, identification of samples, and evaluation
Procedure: List the steps for the lab
Autoclave- to maintain a sterile environment (uses steam)
Growing- fixed incubator 37 C and shaker (growth/ liquid uses shirling method)
Visualizing- microscope
Storing- refrigerator- 4 C
Lab safety: Use PPE- latex or thermal gloves, googles, lab coat (never leave the lab with any of
your PPE).
Notes: Any additional information
,Results:
Summary of the experiment
Lab 2 Notebook
Title: KV02:
Introduction to Microscopy
Objective:
To become familiar with the components of light microscopes and learn how to view a
sample.
Procedure:
Know the parts and components: Eyepiece- (ocular lens) what you look through to see the
sample
Arm or Neck- it provides structural support that connects head to the base.
Objective Lense- lens that provide different magnification levels to see the sample. 0 is the
smallest magnifies by 5x, 10x objective lens= 10 times magnified, 100xs is 100xs magnified.
Stage- platform that the sample is placed in order to see it through the eyepiece.
Stage clips- they secure the slide on the stage
Course focus- larger knob used to adjust the focus of the microscope, used for high
magnification
Fine focus- smaller knob used illuminate the sample for clearer viewing
, Light source- the light bulb used to light up the sample
Base- provides support and stability at the lower part of the microscope.
2. Place the sample on the microscope: Put the slide with the sample on the stage and
use stage clips to keep it in place.
3. choose what magnification you need- begin with lowest magnification to locate the
sample easier. Once located move the magnification up for a more detailed look.
4. adjust to optimize the view- use the course focus to get a better look at the sample.
Use the fine focus to make the small adjustments for a clearer image. Adjust the diaphragm
to regulate the light intensity for optimal clarity and brightness.
Notes:
Basic idea behind microscopy
Similar to magnifying glass (needs similar components)- lens, object, and light.
How to see the sample:
Power on the light source, adjust the knob to help dim it or add more light.
Visualization: type of objectives- dry- 4, 10, and 40x. No other medium is needed to view the
object.
Oil= 100x, oil is needed for imaging. This gives better resolution on higher mags. Add a drop
of immersion oil, helps with light refraction.
When finding small tissue or bacteria start at a higher mag.
When you are uncertain start at the lowest magnification.
Results: The magnification = the power of the objective in the eyepiece.
Lab Notebook 1-9: Complete Updated Solution
Lab 1 Notebook
Title: Introduction to Medical Microbiology
Objective: To establish an organized template for keeping experimental records, procedures,
and results
Cultivation of lab samples, identification of samples, and evaluation
Procedure: List the steps for the lab
Autoclave- to maintain a sterile environment (uses steam)
Growing- fixed incubator 37 C and shaker (growth/ liquid uses shirling method)
Visualizing- microscope
Storing- refrigerator- 4 C
Lab safety: Use PPE- latex or thermal gloves, googles, lab coat (never leave the lab with any of
your PPE).
Notes: Any additional information
,Results:
Summary of the experiment
Lab 2 Notebook
Title: KV02:
Introduction to Microscopy
Objective:
To become familiar with the components of light microscopes and learn how to view a
sample.
Procedure:
Know the parts and components: Eyepiece- (ocular lens) what you look through to see the
sample
Arm or Neck- it provides structural support that connects head to the base.
Objective Lense- lens that provide different magnification levels to see the sample. 0 is the
smallest magnifies by 5x, 10x objective lens= 10 times magnified, 100xs is 100xs magnified.
Stage- platform that the sample is placed in order to see it through the eyepiece.
Stage clips- they secure the slide on the stage
Course focus- larger knob used to adjust the focus of the microscope, used for high
magnification
Fine focus- smaller knob used illuminate the sample for clearer viewing
, Light source- the light bulb used to light up the sample
Base- provides support and stability at the lower part of the microscope.
2. Place the sample on the microscope: Put the slide with the sample on the stage and
use stage clips to keep it in place.
3. choose what magnification you need- begin with lowest magnification to locate the
sample easier. Once located move the magnification up for a more detailed look.
4. adjust to optimize the view- use the course focus to get a better look at the sample.
Use the fine focus to make the small adjustments for a clearer image. Adjust the diaphragm
to regulate the light intensity for optimal clarity and brightness.
Notes:
Basic idea behind microscopy
Similar to magnifying glass (needs similar components)- lens, object, and light.
How to see the sample:
Power on the light source, adjust the knob to help dim it or add more light.
Visualization: type of objectives- dry- 4, 10, and 40x. No other medium is needed to view the
object.
Oil= 100x, oil is needed for imaging. This gives better resolution on higher mags. Add a drop
of immersion oil, helps with light refraction.
When finding small tissue or bacteria start at a higher mag.
When you are uncertain start at the lowest magnification.
Results: The magnification = the power of the objective in the eyepiece.
