BIO 351 - Exam 1 Questions With Complete Solutions
D) mRNA
17. Reverse transcription polymerase chain reaction (PT-PCR)
detects what type of molecule?
A) DNA
B) protein
C) lipids
D) mRNA
E) oligosaccharide polymers
B) meiosis: diplotene I
18. An oogonium enters _____________ embryonically and is
blocked at _____________ until sexual maturity. Fill in blanks.
A) meiosis: metaphase II
B) meiosis: diplotene I
C) mitosis: metaphase II
D) mitosis: diplotene I
A) Transcription of Pax6 in lens, retina, and neural tube not
pancreas
19. The mouse Pax6 gene with its regulatory regions is shown
below. What would be the most likely result if you generated a
targeted mouse mutation (knock-in) that destroyed only the
sequence of the transcription factor binding site
(TGATTGACAG) shown below in bold?
,A) Transcription of Pax6 in lens, retina, and neural tube not
pancreas
B) Transcription of Pax6 in pancreas but not lens, retina and
neural tube
C) No binding of microRNAs
D) No transcription of Pax6 in any tissues
E) Transcription of Pax6 in all tissues
B) A synthetic guide RNA that directs binding of the Cas9
nuclease to specific sites in the genome.
20. The specificity of the engineered CRISPR/Cas9 system for
use in gene editing relies upon which of the following?
A) CRISPR sites only present in the mammalian genome.
B) A synthetic guide RNA that directs binding of the Cas9
nuclease to specific sites in the genome.
C) Targeting genes that have been modified with flanking loxp-
sites ("floxed")
D) Cell specific promoters that drive Cas9 expression only in
select cell types.
, B) Slow block to polyspermy, degradation of CSF, and egg
activation.
21. At fertilization there is a series of Ca2+ (calcium) waves
within the egg. Which of the following occurs in the egg in
response to these waves?
A) A cortical granule excites, capacitation, and the acrosome
reaction.
B) Slow block to polyspermy, degradation of CSF, and egg
activation.
C) Degration of MPF, completion of mitosis, and egg activation.
D) Fast block to polyspermy, degradation of MPF, and
syngamy.
A) heart specific enhancer/promoter, intron, LacZ (beta-
galactosidase) gene, and 3'UTR (untranslated region)
22. From the list below, use the numbers to identify the minimal
elements required to construct a trans gene that will be
expressed in the heart only.
A) heart specific enhancer/promoter, intron, LacZ (beta-
galactosidase) gene, and 3'UTR (untranslated region)
B) heart specific enhancer/promoter, intron, liver specific
promoter, neomycin resistance gene, and thymidine kinase (TK)
C) liver specific promoter, LacZ (beta-galactosidase) gene,
neomycin resistance gene, Gene X genomic DNA
D) heart specific enhancer/promoter, liver specific promoter,
LacZ (beta-galactosidase) gene, and thymidine kinase (TK)
D) mRNA
17. Reverse transcription polymerase chain reaction (PT-PCR)
detects what type of molecule?
A) DNA
B) protein
C) lipids
D) mRNA
E) oligosaccharide polymers
B) meiosis: diplotene I
18. An oogonium enters _____________ embryonically and is
blocked at _____________ until sexual maturity. Fill in blanks.
A) meiosis: metaphase II
B) meiosis: diplotene I
C) mitosis: metaphase II
D) mitosis: diplotene I
A) Transcription of Pax6 in lens, retina, and neural tube not
pancreas
19. The mouse Pax6 gene with its regulatory regions is shown
below. What would be the most likely result if you generated a
targeted mouse mutation (knock-in) that destroyed only the
sequence of the transcription factor binding site
(TGATTGACAG) shown below in bold?
,A) Transcription of Pax6 in lens, retina, and neural tube not
pancreas
B) Transcription of Pax6 in pancreas but not lens, retina and
neural tube
C) No binding of microRNAs
D) No transcription of Pax6 in any tissues
E) Transcription of Pax6 in all tissues
B) A synthetic guide RNA that directs binding of the Cas9
nuclease to specific sites in the genome.
20. The specificity of the engineered CRISPR/Cas9 system for
use in gene editing relies upon which of the following?
A) CRISPR sites only present in the mammalian genome.
B) A synthetic guide RNA that directs binding of the Cas9
nuclease to specific sites in the genome.
C) Targeting genes that have been modified with flanking loxp-
sites ("floxed")
D) Cell specific promoters that drive Cas9 expression only in
select cell types.
, B) Slow block to polyspermy, degradation of CSF, and egg
activation.
21. At fertilization there is a series of Ca2+ (calcium) waves
within the egg. Which of the following occurs in the egg in
response to these waves?
A) A cortical granule excites, capacitation, and the acrosome
reaction.
B) Slow block to polyspermy, degradation of CSF, and egg
activation.
C) Degration of MPF, completion of mitosis, and egg activation.
D) Fast block to polyspermy, degradation of MPF, and
syngamy.
A) heart specific enhancer/promoter, intron, LacZ (beta-
galactosidase) gene, and 3'UTR (untranslated region)
22. From the list below, use the numbers to identify the minimal
elements required to construct a trans gene that will be
expressed in the heart only.
A) heart specific enhancer/promoter, intron, LacZ (beta-
galactosidase) gene, and 3'UTR (untranslated region)
B) heart specific enhancer/promoter, intron, liver specific
promoter, neomycin resistance gene, and thymidine kinase (TK)
C) liver specific promoter, LacZ (beta-galactosidase) gene,
neomycin resistance gene, Gene X genomic DNA
D) heart specific enhancer/promoter, liver specific promoter,
LacZ (beta-galactosidase) gene, and thymidine kinase (TK)
