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BIOL 1500 Lab 4: Osmosis &
Diffusion Lab Camila Lopez
With 100% correct answers
Camila Lopez
N# N02256180
Lab 4: Osmosis & Diffusion Lab
1. Which substances were able to move through the dialysis tubing? How do
you know? What was not able to move through the membrane? For the
substances that moved through the membrane, which direction did they
move? Was this with or against their concentration gradient?
Likely iodine and glucose moved through the dialysis tubing
because they are small molecules. On the contrary starch usually
does not move through because it is too large. Iodine moves into
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the bag (down its gradient) and reacts with starch, changing color.
Glucose moves out into the beaker solution (down its gradient).
2. What data do you have to support that this movement took place?
The color change from blue to black is when iodine and starch
interact. Glucose absorbance data showing glucose outside the bag.
The bar graph shows increasing absorbance in the experimental
group, supporting diffusion.
3. What substance(s) did not move through the membrane? Review your data
and explain whether your data support your answer.
The dye water did not move through the membrane. Its molecular
weight and size is too high to pass through the selectively permeable
membrane.
4. Compare the movement of the substances to their molecular weight. Explain
what may have been the limiting factor for the substance(s) that did not
move through the dialysis tube.
Small molecules like (iodine, glucose, water) diffuse easily. Large
molecules like (starch, sucrose at certain concentrations) are
restricted. The limiting factor is equal to the pore size of dialysis
tubing relative to molecular size
5. If the potato is placed in an isotonic solution, would it gain weight, lose
weight, or have its weight remain the same? Why? Based on your graph and
the trendline, determine the molarity of the potato.
When the potato was placed in an isotonic solution, there no net gain or
loss of mass because the water moving in, was just as much as water
FOR MORE EXAMS
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BIOL 1500 Lab 4: Osmosis &
Diffusion Lab Camila Lopez
With 100% correct answers
Camila Lopez
N# N02256180
Lab 4: Osmosis & Diffusion Lab
1. Which substances were able to move through the dialysis tubing? How do
you know? What was not able to move through the membrane? For the
substances that moved through the membrane, which direction did they
move? Was this with or against their concentration gradient?
Likely iodine and glucose moved through the dialysis tubing
because they are small molecules. On the contrary starch usually
does not move through because it is too large. Iodine moves into
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, FOR MORE EXAMS
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the bag (down its gradient) and reacts with starch, changing color.
Glucose moves out into the beaker solution (down its gradient).
2. What data do you have to support that this movement took place?
The color change from blue to black is when iodine and starch
interact. Glucose absorbance data showing glucose outside the bag.
The bar graph shows increasing absorbance in the experimental
group, supporting diffusion.
3. What substance(s) did not move through the membrane? Review your data
and explain whether your data support your answer.
The dye water did not move through the membrane. Its molecular
weight and size is too high to pass through the selectively permeable
membrane.
4. Compare the movement of the substances to their molecular weight. Explain
what may have been the limiting factor for the substance(s) that did not
move through the dialysis tube.
Small molecules like (iodine, glucose, water) diffuse easily. Large
molecules like (starch, sucrose at certain concentrations) are
restricted. The limiting factor is equal to the pore size of dialysis
tubing relative to molecular size
5. If the potato is placed in an isotonic solution, would it gain weight, lose
weight, or have its weight remain the same? Why? Based on your graph and
the trendline, determine the molarity of the potato.
When the potato was placed in an isotonic solution, there no net gain or
loss of mass because the water moving in, was just as much as water
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