Biochemistry I Module of the MCAT Self Prep eCourse: Lesson 3: Enzym
Kinetics (Pro)
Study online at https://quizlet.com/_4xy9to
1. Lesson 3: Enzyme Kinetics: Lesson 3: Enzyme Kinetics
2. Write the rate law for the following reaction: A+B -> AB: Rate= k [A][B]
Rate is the change in concentration per unit time.
k is the rate constant (depends on the reaction).
[A] and [B] are the concentrations of reactants A and B.
*Struggling to keep your MCAT equations straight? Simply conquer the 100 most important equations using An-
drew's 100 Most Essential Equations Mastery Course @ https://mcatselfprep.com/course/andrews-equation-mas-
tery-course/
3. In Michaelis-Menten Kinetics experiments, which of the following is assumed
to be true?
I. Substrate concentration is constant.
II. Increasing the Enzyme concentration will not change Vmax.
III. The enzymes are saturated at Vmax.
(A) I Only
(B) III Only
(C) I and II Only
(D) I, II, and III: (B) III Only
In Michaelis-Menten Kinetics experiments:
- ENZYME concentration is held constant as we increase the substrate concentration.
- If you were to increase the enzyme concentration, the Vmax would increase.
- The enzymes are saturated at Vmax.
4. In Michaelis-Menten Kinetics experiments, which of the following is assumed
to be true?
I. Our Solutions are behaving ideally.
II. Our constants ([E] and k) are not changing during the experiment.
, Biochemistry I Module of the MCAT Self Prep eCourse: Lesson 3: Enzym
Kinetics (Pro)
Study online at https://quizlet.com/_4xy9to
III. Substrate can be converted into product with or without the enzyme.
(A) I Only
(B) III Only
(C) I and II Only
(D) I, II, and III: (C) I and II Only
In Michaelis-Menten Kinetics experiments, we assume that:
- Our Solutions are behaving ideally.
- Our constants ([E] and k) are not changing during the experiment.
- Substrate CANNOT be converted into product without the enzyme.
5. How can we increase the rate of a reaction assuming the rate constant (k) is
constant?
I. Increase Substrate Concentration
II. Increase Enzyme Concentration
III. Increase Mixed Inhibitor Concentration
(A) I Only
(B) I and II Only
(C) II and III Only
(D) I, II, and III: (B) I and II Only
We can increase the rate of reaction by increasing the substrate or enzyme concentration.
Adding any type of inhibitor will not increase the rate of a reaction.
6. True or false? Adding more catalyst after a catalytic amount has already been
added will increase the rate of reaction.: False. Adding more catalyst after a catalytic amount has
already been added will NOT AFFECT the rate of reaction.
You only need a small amount ("a catalytic amount") of catalyst for it to do its job. Adding more will not do anything.
, Biochemistry I Module of the MCAT Self Prep eCourse: Lesson 3: Enzym
Kinetics (Pro)
Study online at https://quizlet.com/_4xy9to
7. What does it mean if the rate of reaction has reached maximum velocity
(Vmax)?
(A) There are more products than reactants.
(B) The substrates are no longer able to get any closer to the enzymes.
(C) The substrates are no longer able to get in any better of an orientation.
(D) The enzymes no longer have available active sites.: (D) The enzymes no longer have
available active sites.
When the rate of reaction reaches Vmax, it means that the enzymes are saturated and will not be able to react any more
quickly since all active sites are filled up with substrates.
8. If a reaction has reached Vmax, what would happen to the rate of reaction if
we increase the substrate concentration?
(A) It would increase
(B) It would remain the same
(C) It would decrease
(D) It would stop: (B) It would remain the same
Increasing the substrate concentration would have no effect on the rate of the reaction because all active sites are
already occupied at Vmax.
9. What is the one way to increase the Vmax of a reaction?
(A) Increase Substrate Concentration
(B) Increase Enzyme Concentration
(C) Increase Inhibitor Concentration
(D) Increase Product Concentration: (B) Increase Enzyme Concentration
The only way to increase Vmax is by increasing the enzyme concentration.
10. These are the two steps of enzyme catalysis:
E + S => ES => E + P
Kinetics (Pro)
Study online at https://quizlet.com/_4xy9to
1. Lesson 3: Enzyme Kinetics: Lesson 3: Enzyme Kinetics
2. Write the rate law for the following reaction: A+B -> AB: Rate= k [A][B]
Rate is the change in concentration per unit time.
k is the rate constant (depends on the reaction).
[A] and [B] are the concentrations of reactants A and B.
*Struggling to keep your MCAT equations straight? Simply conquer the 100 most important equations using An-
drew's 100 Most Essential Equations Mastery Course @ https://mcatselfprep.com/course/andrews-equation-mas-
tery-course/
3. In Michaelis-Menten Kinetics experiments, which of the following is assumed
to be true?
I. Substrate concentration is constant.
II. Increasing the Enzyme concentration will not change Vmax.
III. The enzymes are saturated at Vmax.
(A) I Only
(B) III Only
(C) I and II Only
(D) I, II, and III: (B) III Only
In Michaelis-Menten Kinetics experiments:
- ENZYME concentration is held constant as we increase the substrate concentration.
- If you were to increase the enzyme concentration, the Vmax would increase.
- The enzymes are saturated at Vmax.
4. In Michaelis-Menten Kinetics experiments, which of the following is assumed
to be true?
I. Our Solutions are behaving ideally.
II. Our constants ([E] and k) are not changing during the experiment.
, Biochemistry I Module of the MCAT Self Prep eCourse: Lesson 3: Enzym
Kinetics (Pro)
Study online at https://quizlet.com/_4xy9to
III. Substrate can be converted into product with or without the enzyme.
(A) I Only
(B) III Only
(C) I and II Only
(D) I, II, and III: (C) I and II Only
In Michaelis-Menten Kinetics experiments, we assume that:
- Our Solutions are behaving ideally.
- Our constants ([E] and k) are not changing during the experiment.
- Substrate CANNOT be converted into product without the enzyme.
5. How can we increase the rate of a reaction assuming the rate constant (k) is
constant?
I. Increase Substrate Concentration
II. Increase Enzyme Concentration
III. Increase Mixed Inhibitor Concentration
(A) I Only
(B) I and II Only
(C) II and III Only
(D) I, II, and III: (B) I and II Only
We can increase the rate of reaction by increasing the substrate or enzyme concentration.
Adding any type of inhibitor will not increase the rate of a reaction.
6. True or false? Adding more catalyst after a catalytic amount has already been
added will increase the rate of reaction.: False. Adding more catalyst after a catalytic amount has
already been added will NOT AFFECT the rate of reaction.
You only need a small amount ("a catalytic amount") of catalyst for it to do its job. Adding more will not do anything.
, Biochemistry I Module of the MCAT Self Prep eCourse: Lesson 3: Enzym
Kinetics (Pro)
Study online at https://quizlet.com/_4xy9to
7. What does it mean if the rate of reaction has reached maximum velocity
(Vmax)?
(A) There are more products than reactants.
(B) The substrates are no longer able to get any closer to the enzymes.
(C) The substrates are no longer able to get in any better of an orientation.
(D) The enzymes no longer have available active sites.: (D) The enzymes no longer have
available active sites.
When the rate of reaction reaches Vmax, it means that the enzymes are saturated and will not be able to react any more
quickly since all active sites are filled up with substrates.
8. If a reaction has reached Vmax, what would happen to the rate of reaction if
we increase the substrate concentration?
(A) It would increase
(B) It would remain the same
(C) It would decrease
(D) It would stop: (B) It would remain the same
Increasing the substrate concentration would have no effect on the rate of the reaction because all active sites are
already occupied at Vmax.
9. What is the one way to increase the Vmax of a reaction?
(A) Increase Substrate Concentration
(B) Increase Enzyme Concentration
(C) Increase Inhibitor Concentration
(D) Increase Product Concentration: (B) Increase Enzyme Concentration
The only way to increase Vmax is by increasing the enzyme concentration.
10. These are the two steps of enzyme catalysis:
E + S => ES => E + P
