Describe how membrane domains (rafts) are formed and their significance
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Regions with high rigidity/cholesterol
Co-localization of proteins with specific TMD's that will have a specific role.
Endosymbiosis Theory
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Eukaryotic cell w/ nucleus took up photosynthetic bacterial cell which
became mitochondria or chloroplast
,How do sterols (like cholesterol) affect membrane fluidity?
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Decrease fluidity, buffer phase transition from gel to fluid
How do unfolded proteins trigger the IRE1 pathway?
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1) Bip unbinds from IRE1 to bind unfolded proteins
2) IRE dimerizes
3) IRE cleaves mRNA signals that come close
Compare/contrast the process of inserting tail-anchored proteins vs. types I-IV C.
Describe the process involved in the insertion of lipid-anchored proteins
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Types 1-IV use sec61 translocon
Tail anchored proteins use Get3-ATP complex which docks onto
membrane, ATP hydrolysis moves protein to Get1/2 - TMD shuttled out into
membrane
Flippase activity experiment
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, 1. Add fluorescent phospholipids
2. Give one sample ATP, give other nothing
3. Add quencher to both
4. See if flippase brought some fluorescently labeled PL's to the inside
- should happen for the sample given ATP
membranes of mitochondria
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Protons pumped back into matrix
O2-->H2O via protons inside matrix
Sphingomyelin vs. Phosphotidyl Choline properties
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Sph longer and more rigid tails, found in rafts
antiporter
Give this one a try later!
Give this one a try later!
Regions with high rigidity/cholesterol
Co-localization of proteins with specific TMD's that will have a specific role.
Endosymbiosis Theory
Give this one a try later!
Eukaryotic cell w/ nucleus took up photosynthetic bacterial cell which
became mitochondria or chloroplast
,How do sterols (like cholesterol) affect membrane fluidity?
Give this one a try later!
Decrease fluidity, buffer phase transition from gel to fluid
How do unfolded proteins trigger the IRE1 pathway?
Give this one a try later!
1) Bip unbinds from IRE1 to bind unfolded proteins
2) IRE dimerizes
3) IRE cleaves mRNA signals that come close
Compare/contrast the process of inserting tail-anchored proteins vs. types I-IV C.
Describe the process involved in the insertion of lipid-anchored proteins
Give this one a try later!
Types 1-IV use sec61 translocon
Tail anchored proteins use Get3-ATP complex which docks onto
membrane, ATP hydrolysis moves protein to Get1/2 - TMD shuttled out into
membrane
Flippase activity experiment
Give this one a try later!
, 1. Add fluorescent phospholipids
2. Give one sample ATP, give other nothing
3. Add quencher to both
4. See if flippase brought some fluorescently labeled PL's to the inside
- should happen for the sample given ATP
membranes of mitochondria
Give this one a try later!
Protons pumped back into matrix
O2-->H2O via protons inside matrix
Sphingomyelin vs. Phosphotidyl Choline properties
Give this one a try later!
Sph longer and more rigid tails, found in rafts
antiporter
Give this one a try later!
