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ACS Biochemistry Final Exam Questions and Correct Solutions 2024 pKa (chapter 3) - Answer -measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - Answer -compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - Answer -dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) - Answer -the ch...

Title: Analysis of the Conservation of S. cerevisiae MET2 Gene in S. pombe Throughout Evolution. Abstract S. cerevisiae and S. pombe are both members of the Ascomycota phylum. The two yeast species diverged from a common ancestor 1 billion years ago. A research project was conducted to investigate the conservation of the S. cerevisiae MET2 gene in S. pombe. The experiments utilized to evaluate MET2 conservation between the two species were spot plating, yeast colony PCR, restriction diges...

Biochemistry- AAMC FLASH CARDS fully solved 2023The Michaelis-Menten Kinetic equation - correct answer Which combination of lipid type and temperature results in the most disordered membrane? - correct answer Lipid with unsaturated acyl at high tempearture Because unsaturated acyl chains do not pack well well together. They are a mess. When you add high temperature they become a hot mess. Because they is more movement in high temps. The side chain of which amino acid is most likey to f...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? correct answers (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. correct answers 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) diges...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? correct answers (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. correct answers 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) diges...

What is an example of a product easily found in nature? - Answer- Antibiotic In a biotechnology lab, what is tap water is best used for? - Answer- Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? - Answer- mRNA How do DNA molecules vary from one species to another? - Answer- Number of genes and non-coding regions. What is agar? - Answer- Solid media on which bacteria grow. What part of an amino acid differs from one to the other? - Answer- R g...

Biochemistry Exam #2 (Module 3) questions and answers 100% verified. (Exam question 21) Dialysis - correct ates small molecules from large molecules (Exam question 30) When a protein is 'salted-in', it precipitates and is removed form a solution. - correct answers.False When we purify protein, we are going against the natural tendency toward disorder (we are fighting against entropy). - correct answers.True When centrifuging sub-cellular structures, what organ...

FL 5 AAMC MCAT EXAM 2024 What is the chemical structure of a component found in four of the five cofactors used by Na+- NQR? -Answer-says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has an uniquinone AND HERE IS IS MENTIONED T...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

What is an example of a product easily found in nature? Antibiotic In a biotechnology lab, what is tap water is best used for? Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? mRNA How do DNA molecules vary from one species to another? Number of genes and non-coding regions. What is agar? Solid media on which bacteria grow. What part of an amino acid differs from one to the other? R group How can colorless prot...