Sds page Study guides, Class notes & Summaries

What is another name for a Western Blot? - ANSWER Immunoblot Technique that allows one to detect and quantify proteins that react with a specific antibody. - ANSWER Western Blot Protein identification on SDS-PAGE is based on comparison to markers of what? - ANSWER Known molecular weights

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

Lead Duties: what is your number one priority and name the two categories - Answer- number one priority is to be a role model for the instructors on your team. Two categories are administrative and clinical duties Lead duties: Administrative duties (name and describe all 5) - Answer- 1) upkeep all program materials including the log box and all documents contained in the log box: -service verification forms - program plans (program title, SDs, responses and targets all current and dated) ...

Medical Biochemistry, UCF questions and answers latest top score. Alanine - correct answers.Ala, A Arginine - correct answers.Arg, R Asparagine - correct answers.Asn, N Aspartic Acid - correct answers.Asp, D Experimental techniques: PCR - correct answers.-can amplify small amounts of DNA Experimental techniques: Western blot - correct answers.-measures protein levels in a sample using antibodies Experimental techniques: Southern blot - correct answers.-de...

MCAT - Lab Techniques (MilesDown)Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, t...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exch...

MCAT - Lab Techniques (MilesDown) Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass,...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample at A260 = 1...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exc...