Bch 162 Questions With Complete Solutions

SDS-Page abbreviated for Correct Answer-Sodium Dodecyl sulfate Polyacylamide Gel Electrophoretic SDS in the presence of protein and heat Correct Answer-wraps around the protein polypeptide backbone and coat it with negative charges to the protein in order to make sure the protein have the same negative charge/mass ion. The shape of the protein is now linearlized and denatured polypeptide How and where does SDS bind to protein Correct Answer-How? by hydrophobic interaction between dodceyl group and sample Where? protein polypeptide backbone schematically and binds to AA sidechain SDS Correct Answer-it is to wrap the protein with negative charges and denature the protein and allow the protein to have the same negative charge/ mass ratio B-meercatoethanol Correct Answer-it is to break any disulfate bond in and between proteins glycerol Correct Answer-add density to the protein; to ensure that the protein gets to the bottom of the gel and the sample do not diffuses into the other lane