Bdna Guides d'étude, Notes de cours & Résumés

Which is required to generate cDNA A. DNA polymerase B. RNase H C. Restriction Enzyme D. Helicase - correct answer B What is the function of Taq Polymerase - correct answer recognize dsDNA You have a 50 μL primer stock at 9pM/μl and want dilute the stock to 900nM. How much stock should you add(what is the dilution factor) - correct answer 2 W...

1 / 14 1. PCR can test for what diseases?: Hep - B Herpes simplex 1 Herpes simplex 2 HIV HPV Epstein-barr Cytomegalovirus Cystic fibrosis Huntingtons Bk virus Fragile X Parovirus 2. qPCR can test for what diseases?: Herpes simplex 1 Herpes simplex 2 Epstein-barr HPV HIV Cytomegalovirus Bk virus VZV 3. RT PCR can test for what diseases?: HIV Influenza Coronavirus Ewing sarcoma Synovial sarcoma Hep - C 4. Multiplex PCR can test for what diseases?: B. pertussis M. pneumo...

What genes would be screened in a lung cancer panel? - correct answer KRAS, EGFR, ALK In a retrovirus, the RNA acts as: - correct answer Genome and mRNA To what does the AcrAB gene product create resistance to? - correct answer Tetracycline What gene is measured following the treatment with imatinib(Gleevec)? - correct answer ...

MB ASCP QUESTIONS AND ANSWERS SCORED A+ What genes would be screened in a lung cancer panel? KRAS, EGFR, ALK In a retrovirus, the RNA acts as: Genome and mRNA To what does the AcrAB gene product create resistance to? Tetracycline What gene is measured following the treatment with imatinib(Gleevec)? BCR/Abl Blood drawn into what type of tube is compatible for genetic testing? K2-EDTA tube Primer dimers are due to what complimentary issue? 3' to 3' complementarity of primer pairs Microsate...

What does barcoding do in Next Generation Sequencing? Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? Beta The 5' capping pr...

What kind of bond do histones have with DNA? - Answer Ionic What amino acids compose histones? - Answer Lysine and Arginine Which lymphoma has t-cell rearrangements? - Answer Sezary Syndrome Acute Promyelocytic leukemia translocation? - Answer t(15,17) Leading strand polymerase: - Answer Delta DNA repair polymerase(s): - Answer Beta or Epsilon Mitochondrial polymerase: - Answer Gamma Molecular beacon design: - Answer (R-sequence-Q) Which method uses visible light? - Answe...

Mock Exam I and II on Molecular Diagnostics [100% PASS] /VERIFIED/ Which of the following techniques uses probe amplification? a) SDA b) NASBA c) Invader d) bDNA *Ans* c) Invader Invader assay is a probe amplification technique, but the others are signal and target amplification techniques. Which of the following techniques uses signal amplification? a) Invader b) Hybrid Capture c) TMA d) SDA *Ans* b) Hybrid capture Hybrid capture is a signal amplification technique, but the othe...

What kind of bond do histones have with DNA? - Answer Ionic What amino acids compose histones? - Answer Lysine and Arginine Which lymphoma has t-cell rearrangements? - Answer Sezary Syndrome Acute Promyelocytic leukemia translocation? - Answer t(15,17) Leading strand polymerase: - Answer Delta DNA repair polymerase(s): - Answer Beta or Epsilon Mitochondrial polymerase: - Answer Gamma Molecular beacon design: - Answer (R-sequence-Q) Which method uses visible light? - Answer Pyrosequencing Lagging...

MB ASCP 2024 Exam Grade A What genes would be screened in a lung cancer panel? - Answer ️️ -KRAS, EGFR, ALK In a retrovirus, the RNA acts as: - Answer ️️ -Genome and mRNA To what does the AcrAB gene product create resistance to? - Answer ️️ -Tetracycline What gene is measured following the treatment with imatinib(Gleevec)? - Answer ️️ - BCR/Abl Blood drawn into what type of tube is compatible for genetic testing? - Answer ️️ -K2-EDTA tube Primer dimers are due to what...

Which PCR method has the highest specificity? A. qPCR B. Touchdown PCR C. Nested PCR D. Two-step PCR Nested PCR Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? A. 5'-ATCTATGTCGGCAATT-3' B. 5'-TTAACGGCTGTATCTA-3' C. 5'-AATTGCCGACATAGAT-3' D. 5'-GAGCACGCTATCTTAT-3' 5'-ATCTATGTCGGCAATT-3' During DNA replication, the 3' -OH of the growing DNA chain attacks which phosphate of an incoming nucleotide? A. Alpha ...