distribution, or sharing without explicit written permission is strictly prohibited.
AQA A Level Biology Required Practicals
RP 01 – Rate of an Enzyme-Controlled Reaction
● Prepare enzyme and substrate solutions
→ Ensures consistent concentrations for fair testing
om
● Label test tubes for different conditions
● Place tubes in water bath to equilibrate
l.c
→ Brings reactants to the desired temperature before reaction starts
● Add enzyme to substrate and start timer
ai
→ Accurately marks reaction start time
● Measure product at fixed intervals
gm
→ Allows calculation of rate over time
● Repeat for different conditions
→ Tests how the variable affects enzyme activity
@
● Repeat trials and average results
72
→ Increases reliability and reduces effect of anomalies
ct
so
tu
lo