PROTEIN CHEMISTRY QUIZ 1
Multiple Choice Questions (MCQs)
1. Which of the following is not a cell disruption technique?
a. Grinding b. Sonication c. Electrophoresis d. Homogenization
2. What is the main purpose of using detergents in cell disruption?
a. To increase protein solubility b. To stabilize protein structure c. To
remove DNA d. To break lipid membranes
3. Which factor does not typically affect protein stability during isolation?
a. pH b. Temperature c. Salt concentration d. Atmospheric pressure
4. Which chromatography technique is based on size?
a. Ion-exchange chromatography b. Gel filtration chromatography c.
Affinity chromatography d. Hydrophobic interaction chromatography
5. Which of the following is used for long-term protein storage?
a. Room temperature b. 4°C c. -20°C to -196°C d. 37°C
6. What is the main advantage of using a baculovirus/insect cell system for
protein expression?
a. High yield of protein b. Simple procedure c. Low-cost d. All of the above
7. Which of the following proteins is typically used as a standard in SDS-
PAGE?
a. Myoglobin b. Haemoglobin c. Bovine serum albumin (BSA) d.
Lysozyme
8. What is the role of protease inhibitors in protein purification?
a. To enhance protein binding to the column b. To prevent protein
degradation c. To denature the protein d. To increase protein solubility
9. Which protein assay is most suitable for measuring low concentrations of
protein?
a. Bradford assay b. BCA assay c. Lowry assay d. UV absorption
10.In isoelectric focusing, a protein with a pI of 7.0 will migrate towards the:
Multiple Choice Questions (MCQs)
1. Which of the following is not a cell disruption technique?
a. Grinding b. Sonication c. Electrophoresis d. Homogenization
2. What is the main purpose of using detergents in cell disruption?
a. To increase protein solubility b. To stabilize protein structure c. To
remove DNA d. To break lipid membranes
3. Which factor does not typically affect protein stability during isolation?
a. pH b. Temperature c. Salt concentration d. Atmospheric pressure
4. Which chromatography technique is based on size?
a. Ion-exchange chromatography b. Gel filtration chromatography c.
Affinity chromatography d. Hydrophobic interaction chromatography
5. Which of the following is used for long-term protein storage?
a. Room temperature b. 4°C c. -20°C to -196°C d. 37°C
6. What is the main advantage of using a baculovirus/insect cell system for
protein expression?
a. High yield of protein b. Simple procedure c. Low-cost d. All of the above
7. Which of the following proteins is typically used as a standard in SDS-
PAGE?
a. Myoglobin b. Haemoglobin c. Bovine serum albumin (BSA) d.
Lysozyme
8. What is the role of protease inhibitors in protein purification?
a. To enhance protein binding to the column b. To prevent protein
degradation c. To denature the protein d. To increase protein solubility
9. Which protein assay is most suitable for measuring low concentrations of
protein?
a. Bradford assay b. BCA assay c. Lowry assay d. UV absorption
10.In isoelectric focusing, a protein with a pI of 7.0 will migrate towards the:
